Correction: Hussain et al. Starvation Protects Hepatocytes from Inflammatory Damage through Paradoxical mTORC1 Signaling. Cells 2023, 12, 1668

Error in Figure 3B

In the original publication [1], there was an error in the published Figure 3B. Due to an unintentional and unnoticed drag-and-drop procedure, the panels “wild-type/CM” and “wild-type/rapamycin” were duplicated. This error was noticed by the authors when reviewing the final online version of the manuscript. The corrected Figure 3 appears below.

Figure 3. Protection against inflammatory damage provided by calorie restriction requires mTORC1 and AMPKα. (A) Western blot of Raptor and Rictor protein levels in cells subjected to siRNA-mediated Rictor knockdown. Quantification of Rictor protein levels normalized to β-actin with ImageJ is shown on the right plot. (B) Cell death assay in AML12 cells that underwent siRNA-mediated knockdown of Raptor or Rictor. Inflammatory stress and starvation treatments were as before. Cell death was evaluated by dual PI/Hoescht33342 live/dead staining. Scale bar: 50 µm. (C) Quantification of data shown in B. (D) Potency of Akt inhibitor VIII in AML12 cells. AML12 cells treated with 10 μM Akt inhibitor VIII for 24 h or left untreated were stimulated with insulin or cytokine mix and processed for immunodetection by Western blotting of activated/phosphorylated and total Akt with the indicated antibodies. (E) Cell death assay of AML12 cells treated with 10 μM Akt inhibitor VIII for 24 h or left untreated prior to exposure to starvation and/or inflammatory cytokines. (F) AML12 cells transfected with siRNAs against mock, AMPKα1 and/or AMPKα2 were exposed or not to overnight starvation (−/+) followed by Western blot detection of total AMPKα. None: no transfection mix or siRNA; Mock: transfection mix with control siRNA with no homology to any known gene sequence. (G) Densitometric quantification of AMPKα levels shown in panel (F). (H) Cell death assay of AML12 cells transfected with a mix of siRNAs against AMPKα1 and AMPKα2 and challenged by starvation and/or inflammatory cytokines as indicated. (I) AML12 cells were treated with AICAR or MHY1485, and cell extracts were subjected to Western blot detection with the indicated antibodies. (J) AML12 cells were treated with AICAR or MHY1485 and exposed to cytokine mix as indicated. Cell death was measured as before. (K) Quantification of cell death data in panel (J). Cell death data are presented as mean ± SEM; two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test was performed. * indicates p < 0.05, ns indicates non-significance.

Figure 3. Protection against inflammatory damage provided by calorie restriction requires mTORC1 and AMPKα. (A) Western blot of Raptor and Rictor protein levels in cells subjected to siRNA-mediated Rictor knockdown. Quantification of Rictor protein levels normalized to β-actin with ImageJ is shown on the right plot. (B) Cell death assay in AML12 cells that underwent siRNA-mediated knockdown of Raptor or Rictor. Inflammatory stress and starvation treatments were as before. Cell death was evaluated by dual PI/Hoescht33342 live/dead staining. Scale bar: 50 µm. (C) Quantification of data shown in B. (D) Potency of Akt inhibitor VIII in AML12 cells. AML12 cells treated with 10 μM Akt inhibitor VIII for 24 h or left untreated were stimulated with insulin or cytokine mix and processed for immunodetection by Western blotting of activated/phosphorylated and total Akt with the indicated antibodies. (E) Cell death assay of AML12 cells treated with 10 μM Akt inhibitor VIII for 24 h or left untreated prior to exposure to starvation and/or inflammatory cytokines. (F) AML12 cells transfected with siRNAs against mock, AMPKα1 and/or AMPKα2 were exposed or not to overnight starvation (−/+) followed by Western blot detection of total AMPKα. None: no transfection mix or siRNA; Mock: transfection mix with control siRNA with no homology to any known gene sequence. (G) Densitometric quantification of AMPKα levels shown in panel (F). (H) Cell death assay of AML12 cells transfected with a mix of siRNAs against AMPKα1 and AMPKα2 and challenged by starvation and/or inflammatory cytokines as indicated. (I) AML12 cells were treated with AICAR or MHY1485, and cell extracts were subjected to Western blot detection with the indicated antibodies. (J) AML12 cells were treated with AICAR or MHY1485 and exposed to cytokine mix as indicated. Cell death was measured as before. (K) Quantification of cell death data in panel (J). Cell death data are presented as mean ± SEM; two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test was performed. * indicates p < 0.05, ns indicates non-significance.

The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.