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Open AccessArticle

Physicochemical Characterization and Immunomodulatory Activity of a Novel Acid Polysaccharide from Solanum muricatum

by Heng Yue 1,2, Qianqian Xu 1,2, Xianheng Li 1,2, Jeevithan Elango 1,2, Wenhui Wu 1,2,* and Jianfeng Xu 1,2,*
1
Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2
Quality Supervision, Inspection and Testing Center for Cold Storage and Refrigeration Equipment, Ministry of Agriculture, Shanghai 201306, China
*
Authors to whom correspondence should be addressed.
Polymers 2019, 11(12), 1972; https://doi.org/10.3390/polym11121972
Received: 10 October 2019 / Revised: 11 November 2019 / Accepted: 18 November 2019 / Published: 30 November 2019
(This article belongs to the Collection Polysaccharides)
To investigate the structure and immunomodulatory activity of polysaccharide from Solanum muricatum, a novel acid polysaccharide named SMP-3a was purified from Solanum muricatum pulp through DEAE-52 cellulose column and Sephadex G-200 chromatography. Monosaccharide composition analysis showed that SMP-3a was mainly composed of rhamnose, arabinose, galactose, and galacturonic acid with the molar ratio of 1.09:2.64:1.54:1. The average molecular weight was found to be 227 kDa by high performance gel permeation chromatography (HPGPC). Thermal studies revealed the SMP-3a was a thermally stable polymer. Based on the results of methylation and NMR analysis, the backbone chain of SMP-3a was composed of →2)-α-l-Rhap-(1→, →4)-α-d-GalpA-(1→ and →4)-α-d-Galp-(1→. The side chain was consisted of α-l-Araf-(1→ and →5)-α-l-Araf-(1→. Immunomodulatory assay indicated that SMP-3a could significantly promote the proliferation of macrophages and stimulate the secretion of cytokines, including TNF-α, IL-1β, and IL-6. Our results suggested that SMP-3a could be used as a novel potential immunomodulatory agent in functional food. View Full-Text
Keywords: polysaccharide; Solanum muricatum; purification; structural characterization; immunomodulatory activity polysaccharide; Solanum muricatum; purification; structural characterization; immunomodulatory activity
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MDPI and ACS Style

Yue, H.; Xu, Q.; Li, X.; Elango, J.; Wu, W.; Xu, J. Physicochemical Characterization and Immunomodulatory Activity of a Novel Acid Polysaccharide from Solanum muricatum. Polymers 2019, 11, 1972.

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