Electrochemical MIP Sensor for Butyrylcholinesterase
by
Goksu Ozcelikay 1,2, Sevinc Kurbanoglu 1, Xiaorong Zhang 2, Cagla Kosak Soz 3
, Ulla Wollenberger 2, Sibel A. Ozkan 1, Aysu Yarman 2,* and Frieder W. Scheller 2,*
Goksu Ozcelikay 1,2, Sevinc Kurbanoglu 1, Xiaorong Zhang 2, Cagla Kosak Soz 3, Ulla Wollenberger 2, Sibel A. Ozkan 1, Aysu Yarman 2,* and Frieder W. Scheller 2,*
1
Faculty of Pharmacy, Department of Analytical Chemistry, Ankara University, Tandogan, Ankara 06560, Turkey
2
Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany
3
Faculty of Science, Material Science and Technologies, Turkish-German University, Sahinkaya Cad. No. 86, Beykoz, Istanbul 34820, Turkey
*
Authors to whom correspondence should be addressed.
Polymers 2019, 11(12), 1970; https://doi.org/10.3390/polym11121970
Received: 16 October 2019 / Revised: 21 November 2019 / Accepted: 27 November 2019 / Published: 30 November 2019
(This article belongs to the Special Issue Polymers for Chemosensing II)
Molecularly imprinted polymers (MIPs) mimic the binding sites of antibodies by substituting the amino acid-scaffold of proteins by synthetic polymers. In this work, the first MIP for the recognition of the diagnostically relevant enzyme butyrylcholinesterase (BuChE) is presented. The MIP was prepared using electropolymerization of the functional monomer o-phenylenediamine and was deposited as a thin film on a glassy carbon electrode by oxidative potentiodynamic polymerization. Rebinding and removal of the template were detected by cyclic voltammetry using ferricyanide as a redox marker. Furthermore, the enzymatic activity of BuChE rebound to the MIP was measured via the anodic oxidation of thiocholine, the reaction product of butyrylthiocholine. The response was linear between 50 pM and 2 nM concentrations of BuChE with a detection limit of 14.7 pM. In addition to the high sensitivity for BuChE, the sensor responded towards pseudo-irreversible inhibitors in the lower mM range.
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Keywords:
molecularly imprinted polymers; biomimetic sensors; butyrylcholinesterase; o-phenylenediamine; rivastigmine
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MDPI and ACS Style
Ozcelikay, G.; Kurbanoglu, S.; Zhang, X.; Kosak Soz, C.; Wollenberger, U.; Ozkan, S.A.; Yarman, A.; Scheller, F.W. Electrochemical MIP Sensor for Butyrylcholinesterase. Polymers 2019, 11, 1970.
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