Next Article in Journal
Targeted siRNA Nanoparticles for Mammary Carcinoma Therapy
Next Article in Special Issue
Frequent Occurrence of NRAS and BRAF Mutations in Human Acral Naevi
Previous Article in Journal
The Head and Neck Squamous Cell Carcinoma Microenvironment as a Potential Target for Cancer Therapy
Previous Article in Special Issue
A Simplified Genomic Profiling Approach Predicts Outcome in Metastatic Colorectal Cancer
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Cancers
Volume 11
Issue 4
10.3390/cancers11040441
cancers-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Integrated Somatic and Germline Whole-Exome Sequencing Analysis in Women with Lung Cancer after a Previous Breast Cancer

by
Simona Coco
1,*,†,
Silvia Bonfiglio
2,†,
Davide Cittaro
2,†,
Irene Vanni
1,3,
Marco Mora
4,
Carlo Genova
1,3,
Maria Giovanna Dal Bello
1,
Simona Boccardo
1,
Angela Alama
1,
Erika Rijavec
1,
Claudio Sini
1,
Valeria Rossella
5,
Giulia Barletta
1,
Federica Biello
1,
Anna Truini
1,
Cristina Bruzzo
1,
Maurizio Gallo
3,
Dejan Lazarevic
2,
Alberto Ballestrero
3 and
Francesco Grossi
6
1
Lung Cancer Unit, IRCCS Ospedale Policlinico San Martino, 16132 Genoa, Italy
2
Centre for Translational Genomics and Bioinformatics, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy
3
Department of Internal Medicine and Medical Specialties (DIMI), University of Genoa, IRCCS Ospedale Policlinico San Martino, 16132 Genoa, Italy
4
Department of Pathology, IRCCS Ospedale Policlinico San Martino, 16132 Genoa, Italy
5
Swiss Stem Cell Biotech, 6830 Vacallo, Switzerland
6
UOC Oncologia Medica Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to the work.
Cancers 2019, 11(4), 441; https://doi.org/10.3390/cancers11040441
Submission received: 8 February 2019 / Revised: 12 March 2019 / Accepted: 25 March 2019 / Published: 28 March 2019
(This article belongs to the Special Issue Application of Next-Generation Sequencing in Cancers)
Browse Figures
Review Reports Versions Notes

Abstract

Women treated for breast cancer (BC) are at risk of developing secondary tumors, such as lung cancer (LC). Since rare germline variants have been linked to multiple cancer development, we hypothesized that BC survivors might be prone to develop LC as a result of harboring rare variants. Sixty patients with LC with previous BC (the study population; SP) and 53 women with either BC or LC and no secondary cancer (control population; CP) were enrolled. Whole exome sequencing was performed in both tumors and unaffected tissues from 28/60 SP patients, and in germline DNA from 32/53 CP. Candidate genes were validated in the remaining individuals from both populations. We found two main mutational signature profiles: S1 (C>T) in all BCs and 16/28 LCs, and S2 (C>A) which is strongly associated with smoking, in 12/28 LCs. The burden test over rare germline variants in S1-LC vs CP identified 248 genes. Validation confirmed GSN as significantly associated with LC in never-smokers. In conclusion, our data suggest two signatures involved in LC onset in women with previous BC. One of these signatures is linked to smoking. Conversely, regardless of smoking habit, in a subgroup of BC survivors genetic susceptibility may contribute to LC risk.
Keywords: breast cancer; lung cancer; multiple cancer susceptibility; genetic predisposition; exome sequencing breast cancer; lung cancer; multiple cancer susceptibility; genetic predisposition; exome sequencing

Share and Cite

MDPI and ACS Style

Coco, S.; Bonfiglio, S.; Cittaro, D.; Vanni, I.; Mora, M.; Genova, C.; Dal Bello, M.G.; Boccardo, S.; Alama, A.; Rijavec, E.; et al. Integrated Somatic and Germline Whole-Exome Sequencing Analysis in Women with Lung Cancer after a Previous Breast Cancer. Cancers 2019, 11, 441. https://doi.org/10.3390/cancers11040441

AMA Style

Coco S, Bonfiglio S, Cittaro D, Vanni I, Mora M, Genova C, Dal Bello MG, Boccardo S, Alama A, Rijavec E, et al. Integrated Somatic and Germline Whole-Exome Sequencing Analysis in Women with Lung Cancer after a Previous Breast Cancer. Cancers. 2019; 11(4):441. https://doi.org/10.3390/cancers11040441

Chicago/Turabian Style

Coco, Simona, Silvia Bonfiglio, Davide Cittaro, Irene Vanni, Marco Mora, Carlo Genova, Maria Giovanna Dal Bello, Simona Boccardo, Angela Alama, Erika Rijavec, and et al. 2019. "Integrated Somatic and Germline Whole-Exome Sequencing Analysis in Women with Lung Cancer after a Previous Breast Cancer" Cancers 11, no. 4: 441. https://doi.org/10.3390/cancers11040441

APA Style

Coco, S., Bonfiglio, S., Cittaro, D., Vanni, I., Mora, M., Genova, C., Dal Bello, M. G., Boccardo, S., Alama, A., Rijavec, E., Sini, C., Rossella, V., Barletta, G., Biello, F., Truini, A., Bruzzo, C., Gallo, M., Lazarevic, D., Ballestrero, A., & Grossi, F. (2019). Integrated Somatic and Germline Whole-Exome Sequencing Analysis in Women with Lung Cancer after a Previous Breast Cancer. Cancers, 11(4), 441. https://doi.org/10.3390/cancers11040441

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop