Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor

Martínez-Pérez, Paula; Gómez-Gómez, Maribel; Angelova, Todora; Griol, Amadeu; Hurtado, Juan; Bellieres, Laurent; García-Rupérez, Jaime

doi:10.3390/mi11050464

Open AccessArticle

Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor

by

Paula Martínez-Pérez

,

Maribel Gómez-Gómez

,

Todora Angelova

,

Amadeu Griol

,

Juan Hurtado

,

Laurent Bellieres

and

Jaime García-Rupérez

^*

Nanophotonics Technology Center, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain

^*

Author to whom correspondence should be addressed.

Micromachines 2020, 11(5), 464; https://doi.org/10.3390/mi11050464

Submission received: 8 April 2020 / Revised: 23 April 2020 / Accepted: 27 April 2020 / Published: 28 April 2020

(This article belongs to the Special Issue Photonic Crystal Microsensors)

Download

Browse Figures

Versions Notes

Abstract

Thrombin generation is a complex and finely regulated pathway that provokes dynamical changes of thrombin concentration in blood when a vascular injury occurs. In order to characterize the initiation phase of such process, when thrombin concentration is in the nM range, a label-free optical aptasensor is proposed here. This aptasensor combines a 1D photonic crystal structure consisting of a silicon corrugated waveguide with thrombin binding aptamers on its surface as bioreceptors. As a result, this aptasensor has been demonstrated to specifically detect thrombin concentrations ranging from 270 pM to 27 nM with an estimated detection limit of 33.5 pM and a response time of ~2 min. Furthermore, it has also been demonstrated that this aptasensor is able to continuously respond to consecutive increasing concentrations of thrombin and to detect binding events as they occur. All these features make this aptasensor a good candidate to continuously study how thrombin concentration progressively increases during the initiation phase of the coagulation cascade.

Keywords: photonic crystal; photonic bandgap; optical biosensor; aptasensor; label-free; thrombin

Graphical Abstract

Share and Cite

MDPI and ACS Style

Martínez-Pérez, P.; Gómez-Gómez, M.; Angelova, T.; Griol, A.; Hurtado, J.; Bellieres, L.; García-Rupérez, J. Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor. Micromachines 2020, 11, 464. https://doi.org/10.3390/mi11050464

AMA Style

Martínez-Pérez P, Gómez-Gómez M, Angelova T, Griol A, Hurtado J, Bellieres L, García-Rupérez J. Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor. Micromachines. 2020; 11(5):464. https://doi.org/10.3390/mi11050464

Chicago/Turabian Style

Martínez-Pérez, Paula, Maribel Gómez-Gómez, Todora Angelova, Amadeu Griol, Juan Hurtado, Laurent Bellieres, and Jaime García-Rupérez. 2020. "Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor" Micromachines 11, no. 5: 464. https://doi.org/10.3390/mi11050464

APA Style

Martínez-Pérez, P., Gómez-Gómez, M., Angelova, T., Griol, A., Hurtado, J., Bellieres, L., & García-Rupérez, J. (2020). Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor. Micromachines, 11(5), 464. https://doi.org/10.3390/mi11050464

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI