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Influence of Pulsed He–Ne Laser Irradiation on the Red Blood Cell Interaction Studied by Optical Tweezers
Open AccessArticle

Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

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Optoelectronics and Measurement Techniques Research Unit, University of Oulu, 90014 Oulu, Finland
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Nanobiotechnology Laboratory, St. Petersburg Academic University, St. Petersburg 194021, Russia
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RASA Center in St. Petersburg, Peter the Great St. Petersburg Polytechnic University, St. Petersburg 195251, Russia
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Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Laboratory of Developmental Biology, University of Oulu, 90014 Oulu, Finland
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InfoTech Oulu, Borealis Biobank of Northern Finland, Oulu University Hospital, University of Oulu, 90014 Oulu, Finland
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Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, Tomsk 634050, Russia
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Institute of Engineering Physics for Biomedicine (PhysBio), National Research Nuclear University MEPhI, Moscow 115409, Russia
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Aston Institute of Materials Research, School of Engineering and Applied Science, Aston University, Birmingham B4 7ET, UK
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School of Life & Health Sciences, Aston University, Birmingham B4 7ET, UK
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Authors to whom correspondence should be addressed.
Micromachines 2020, 11(1), 19; https://doi.org/10.3390/mi11010019
Received: 16 November 2019 / Revised: 11 December 2019 / Accepted: 17 December 2019 / Published: 23 December 2019
(This article belongs to the Special Issue Optical Trapping and Manipulation: From Fundamentals to Applications)
In the framework of novel medical paradigm the red blood cells (RBCs) have a great potential to be used as drug delivery carriers. This approach requires an ultimate understanding of the peculiarities of mutual interaction of RBC influenced by nano-materials composed the drugs. Optical tweezers (OT) is widely used to explore mechanisms of cells’ interaction with the ability to trap non-invasively, manipulate and displace living cells with a notably high accuracy. In the current study, the mutual interaction of RBC with polymeric nano-capsules (NCs) is investigated utilizing a two-channel OT system. The obtained results suggest that, in the presence of NCs, the RBC aggregation in plasma satisfies the ‘cross-bridges’ model. Complementarily, the allocation of NCs on the RBC membrane was observed by scanning electron microscopy (SEM), while for assessment of NCs-induced morphological changes the tests with the human mesenchymal stem cells (hMSC) was performed. The combined application of OT and advanced microscopy approaches brings new insights into the conception of direct observation of cells interaction influenced by NCs for the estimation of possible cytotoxic effects. View Full-Text
Keywords: polymeric nanocapsules; red blood cells; optical tweezers; SEM; optical microscopy; cytotoxicity; human mesenchymal stem cells polymeric nanocapsules; red blood cells; optical tweezers; SEM; optical microscopy; cytotoxicity; human mesenchymal stem cells
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Avsievich, T.; Tarakanchikova, Y.; Zhu, R.; Popov, A.; Bykov, A.; Skovorodkin, I.; Vainio, S.; Meglinski, I. Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy. Micromachines 2020, 11, 19.

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