Search Results (218)

Single-cell adhesion assays can be divided into studies on attachment and detachment events, and several methods that enable the characterization of both processes have been established in the past. Due to their low invasiveness, label-free principles, and contactless operation, optical methods are especially beneficial for this purpose. Historically, optical tweezers (OTs) have been used to explore single-cell detachment events, allowing for the precise determination of minute physical forces. However, it has been noted that OTs can also be used to study single-cell attachment dynamics, including the evaluation of minimum cell-to-cell contact times necessary to establish a stable adhesive bond. Here, we provide a step-by-step protocol to effectively evaluate minute changes in the adhesion of single leukemia–lymphoma cells using optical tweezers with low laser intensities. This serves as a valuable in vitro model to determine the effects of physical and chemical factors on the adhesive properties of leukemia–lymphoma (LL) cells. Full article

We propose a two-qubit phase gate based on trapped ions that uses fast electric field pulses and spin-dependent local traps generated by optical tweezers. The phases are engineered by spin-dependent coherent evolution, interspersed with momentum kicks. We derive a set of commensurability conditions and expressions for the spin-dependent accumulated phase that, when satisfied, realize the target two-qubit phase gate within tens of microseconds. We study the scalability of our proposal in larger-ion crystals and demonstrate the existence of solutions with up to four ions. Gates in larger crystals should also be possible but will require more commensurability conditions to be fulfilled. Full article

Micromotors play a crucial role in microsystems technology, with applications in nanoparticle propulsion, targeted drug delivery, and biosensing. Optical field propulsion, particularly optical tweezers (OTs), enables precise, noncontact control but traditionally relies on Gaussian traps, which require preprogramming and offer limited rotational control. Here, we introduce a micromotor driven by optical vortex beams, utilizing phase gradients to generate optical torque. This eliminates preprogramming and enables real-time control over rotation and positioning. Using this method, we design red blood cell (RBC)-based micromotors for targeted cellular debris collection in liquid environments. Our findings provide a versatile strategy for micro-/nano-object manipulation with potential applications in biomedicine and precision transport. Full article

The research on high-order transverse modes in lasers is a subject as old as the laser itself and has been largely abandoned. However, recently several studies have demonstrated an interest in using, instead of the usual Gaussian beam, a radial Laguerre–Gauss LG_p0 beam, as, for instance, one can observe a strong improvement, for a given power, in the longitudinal and radial forces in optical tweezers illuminated by a LG_p0 beam instead of the usual Gaussian beam. Since in most commercial lasers, the delivered laser beam is Gaussian, we therefore think it opportune to consider the problems of forcing a laser to oscillate individually on a higher-order transverse LG_p0 mode. We propose a comprehensive analysis of the effects of an intra-cavity phase or amplitude mask on the fundamental mode of a plano-concave cavity. In particular, we discuss the best choice of parameters favouring the fundamental mode of a pure radial Laguerre–Gauss LG_p0 model. Full article

Background: Acute myeloid leukemia (AML) is a heterogeneous disease highly resistant to chemotherapeutic agents. Leukemia stem cells (LSCs) can enter a dormant state and avoid apoptosis in the protective niche of the bone marrow (BM) microenvironment. Moreover, bone marrow stromal cells protect leukemia cells by promoting pro-survival signaling pathways and drug resistance. Therefore, attenuating interactions between leukemia cells and BM cells may have a positive therapeutic effect. Objectives: In this work, we hypothesized that sondages may inhibit the adhesion of leukemia cells to the bone marrow by inhibiting the Hedgehog (Hh) signaling pathway. The Hedgehog pathway is a key therapeutic target in AML due to its role in leukemic cell growth and survival. Methods: We investigated the effects of sonidegib on the adhesion of individual OCI-AML3 cells to a bone marrow stromal spheroid derived from the HS-5 cell line. For this purpose, we precisely determined the minimum cell-to-cell adhesion time using optical tweezers under normoxic (21% of O₂) and hypoxic (1% of O₂) conditions. Results: Our results demonstrated that sonidegib significantly increased the minimum cell-to-cell adhesion time necessary for leukemic cells to establish adhesive bonds with bone marrow stromal cells, thereby indicating a reduction in their adhesive properties. Additionally, we showed that sonidegib is particularly effective at hypoxic oxygen concentrations. Conclusions: The results obtained in this study suggest that sonidegib, through its modulation of the Hedgehog signaling pathway, holds promise as a potential therapeutic approach to target leukemic cell adhesion within the bone marrow microenvironment. Full article

Defect-free atom arrays provide new possibilities for exploring exotic quantum phenomena and realizing quantum computing. However, quickly and efficiently preparing defect-free atom arrays poses challenges. This paper proposes an innovative parallel rearrangement method, namely the parallel compression filling algorithm (PCFA), wherein multiple movable optical tweezers operate simultaneously. By limiting the shape of the initial loading, the method reduces movement complexity. The simulation comparisons show that this algorithm is more efficient in preparing defect-free atom arrays and can also be applied to the generation of other periodic structure arrays. The simulation results show that, in most cases, preparing a defect-free array of 400 atoms requires no more than 30 steps. Full article

An ultra-thin spiral diffractive lens (SDL) was fabricated by using focused ion beam milling on a fiber end-facet coated with a 100 nm thick Au film. Focusing vortex beams (FVBs) were successfully excited by the SDLs due to the coherent superposition of diffracted waves and their azimuth dependence of the phase accumulated from the spiral aperture to the beam axis. The polarization and phase characteristics of the FVBs were experimentally investigated. Results show that the input beams with various polarization states were converted to FVBs, whose polarization states were the same as those of the input beams. Furthermore, the focal length of the SDL and the in-tensity and phase distribution at the focus spot of the FVBs were numerically simulated by the FDTD method in the ultra-wide near-infrared waveband from 1300 nm to 1800 nm. The focal length was tuned from 21.8 μm to 14.7 μm, the intensity profiles exhibited a doughnut-like shape, and the vortex phase was converted throughout the broadband range. The devices are expected to be candidates for widespread applications including optical communications, optical imaging, and optical tweezers. Full article

Nanoscale resonant devices based on optical tweezers are widely used in the field of precision sensing. In the process of driving the nanoresonator based on the Coulomb force, the real-time, precise regulation of the charge carried by the charged resonator is essential for continuous manipulation. However, the accuracy of the existing charge measurement methods for levitated particles is low, and these methods cannot meet the needs of precision sensing. In this study, a novel net charge measurement protocol for levitated particles based on spatial speed statistics is proposed. High-precision mass measurement based on Maxwell’s rate distribution law is the basis for improving the accuracy of charge measurement, and accurate measurement of net charge can be achieved by periodic electric field driving. The error of net charge measurement is less than 7.3% when the pressure is above 0.1 mbar, while it can be less than 0.76% at 10 mbar. This proposed method features real-time, high-precision, non-destructive, and in situ measurement of the net charge of particles in the medium vacuum, which provides new solutions for practical problems in the fields of high-precision sensing and nano-metrology based on levitated photodynamics. Full article

The analysis of nucleic acid structures, topologies, nano-mechanics and interactions with ligands and other biomacromolecules (most notably proteins) at the single molecule level has become a fundamental topic in molecular biophysics over the last two decades. Techniques such as molecular tweezers, single-molecule fluorescence resonance energy transfer, and atomic force microscopy have enabled us to disclose an unprecedented insight into the mechanisms governing gene replication, transcription and regulation. In this minireview, we survey the main working principles and discuss technical caveats of the above techniques, using as a fil-rouge the history of their achievements in dissecting G-quadruplexes. The revised literature offers a clear example of the superior ability of single-molecule techniques with respect to ensemble techniques to unveil the structural and functional diversity of the several polymorphs corresponding to a single G-quadruplex folding sequence, thus shedding new light on the extreme complexity of these fascinating non-Watson–Crick structures. Full article

Transverse mode control of laser intracavity oscillation is crucial for generating high-purity cylindrical vector beams (CVBs). We utilized the mode conversion and mode selection properties of two-mode long-period fiber gratings (TM-LPFGs) and two-mode fiber Bragg gratings (TM-FBGs) to achieve intracavity hybrid-mode oscillations of LP₀₁ and LP₁₁ from an all-few-mode fiber laser. A mode-locked pulse output with a repetition rate of 12.46 MHz and a signal-to-noise ratio of 53 dB was achieved with a semiconductor saturable absorber mirror (SESAM) for mode-locking, at a wavelength of 1550.32 nm. The 30 dB spectrum bandwidth of the mode-locked pulse was 0.13 nm. Furthermore, a high-purity CVB containing radially polarized and azimuthally polarized LP₁₁ modes was generated. The purity of the obtained CVB was greater than 99%. The high-purity CVB pulses have great potential for applications in optical tweezers, high-speed mode-division multiplexing communication, and more. Full article

It is well established that the interaction of light with matter generally imparts mechanical forces and associated torques. Where laser light is involved, such effects can be deployed for the microscale and nanoscale manipulation of matter, giving rise to notable applications in wide areas of physics, chemistry, and the life sciences. The diversity of applications is enriched not only by the complexity of the constitution of matter, but also by the structure of the laser light itself, according to its polarisation, amplitude, and phase. This article reviews in comprehensive terms the origins of laser-based optical forces and their manifestations in a variety of current contexts and applications. Full article

Usually, optical tweezers for trapping atoms or nanoparticles are based on the focusing of a Gaussian laser beam (GB). The optical trap is characterised by its longitudinal stability (LS), expressed as the ratio of the backward axial gradient and the forward scattering forces. Replacing the GB with a LG_p0 beam (one central peak surrounded by p rings) does not improve the LS because the on-axis intensity distribution is the same whatever the mode order p. However, it has been recently demonstrated that a restructured LG_p0 beam can improve greatly the LS. In this paper, we consider the restructuring of a LG_p0 beam when passing through a simple binary diffracting optical element called a circular π-plate (CPP). For a particular radius of the dephasing zone of the CPP, it is found that the LS is multiplied by a factor corresponding to a relative increase of about 220% to 320%. Full article

Optical vortex lattices (OVLs) with diverse modes show potential for a wide range of applications, such as high-capacity optical communications, optical tweezers, and optical measurements. However, vortices in typical regulated OVLs often exhibit irregular shapes, such as being narrow and elongated. The resulting increase in asymmetry negatively impacts the efficiency of particle trapping. Additionally, the vortex radii expand with an increase in topological charge (TC), limiting the TC value of the vortices and hindering their ability to fully utilize orbital angular momentum (OAM). Herein, we propose an alternative approach to custom OVLs using off-axis techniques combined with amplitude modulation. Amplitude modulation enables the precise generation of an OVL with perfect vortex properties, known as a perfect off-axis OVL. Further, the number of vortices in the perfect off-axis OVL, the off-axis distances, and the TC can be freely modulated while maintaining a circular mode. This unique OVL will promote new applications, such as the complex manipulation of multi-particle systems and optical communication based on OAM. Full article

The study aims to determine how chitosan impacts pectin hydrogel’s ability to attach peritoneal leukocytes, activate complement, induce hemolysis, and adsorb blood proteins. The hydrogels PEC-Chi0, PEC-Chi25, PEC-Chi50, and PEC-Chi75 were prepared by placing a mixture solution of 4% pectin and 4% chitosan in a ratio of 4:0, 3:1, 2:2, and 1:3 in a solution of 1.0 M CaCl₂. Chitosan was found to modify the mechanical properties of pectin–calcium hydrogels, such as hardness and cohesiveness-to-adhesiveness ratio. Chitosan in the pectin–calcium hydrogel caused pH-sensitive swelling in Hanks’ solution. The PEC-Chi75 hydrogel was shown to adsorb serum proteins at pH 7.4 to a greater extent than other hydrogels. PEC-Chi75’s strong adsorption capacity was related to lower peritoneal leukocyte adherence to its surface when compared to other hydrogels, showing improved biocompatibility. Using the optical tweezers approach, it was shown that the force of interaction between pectin–chitosan hydrogels and plasma proteins increased from 10 to 24 pN with increasing chitosan content from 0 to 75%. Thus, the properties of pectin–calcium hydrogel, which determine interactions with body tissues after implantation, are improved by the addition of chitosan, making pectin–chitosan hydrogel a promising candidate for smart biomaterial development. Full article