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Article

An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction

1
The Nano Science and Technology (NSNT) Program, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
2
Shenzhen Shineway Hi-Tech Co., Ltd., Shenzhen 518112, China
3
Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
4
Guangzhou HKUST Fok Ying Tung Research Institute, Nansha, Guangzhou 511458, China
5
Department of Ocean Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
6
Materials Genome Institute, University of Shanghai, Shanghai 200444, China
*
Authors to whom correspondence should be addressed.
Micromachines 2019, 10(3), 204; https://doi.org/10.3390/mi10030204
Received: 7 February 2019 / Revised: 8 March 2019 / Accepted: 20 March 2019 / Published: 22 March 2019
(This article belongs to the Special Issue Microfluidics for Cells and Other Organisms)
The result of molecular diagnostic and detection greatly dependent on the quality and integrity of the isolated nucleic acid. In this work, we developed an automated miniaturized nucleic acid extraction device based on magnetic beads method, consisting of four components including a sample processing disc and its associated rotary power output mechanism, a pipetting module, a magnet module and an external central controller to enable a customizable and automated robust nucleic acid sample preparation. The extracted nucleic acid using 293T cells were verified using real-time polymerase chain reaction (PCR) and the data implies a comparable efficiency to a manual process, with the advantages of performing a flexible, time-saving (~10 min), and simple nucleic acid sample preparation. View Full-Text
Keywords: sample preparation; nucleic acid; DNA; RNA sample preparation; nucleic acid; DNA; RNA
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MDPI and ACS Style

Tong, R.; Zhang, L.; Hu, C.; Chen, X.; Song, Q.; Lou, K.; Tang, X.; Chen, Y.; Gong, X.; Gao, Y.; Wen, W. An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction. Micromachines 2019, 10, 204. https://doi.org/10.3390/mi10030204

AMA Style

Tong R, Zhang L, Hu C, Chen X, Song Q, Lou K, Tang X, Chen Y, Gong X, Gao Y, Wen W. An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction. Micromachines. 2019; 10(3):204. https://doi.org/10.3390/mi10030204

Chicago/Turabian Style

Tong, Rui, Lijuan Zhang, Chuandeng Hu, Xuee Chen, Qi Song, Kai Lou, Xin Tang, Yongsheng Chen, Xiuqing Gong, Yibo Gao, and Weijia Wen. 2019. "An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction" Micromachines 10, no. 3: 204. https://doi.org/10.3390/mi10030204

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