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Micromachines
Volume 10
Issue 12
10.3390/mi10120837
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Article

Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA

by
Yu-Ting Lai
1,
Chia-Hsin Tsai
2,
Ju-Chun Hsu
2 and
Yen-Wen Lu
1,*
1
Department of Biomechatronics Engineering, National Taiwan University, Taipei 10016, Taiwan
2
Department of Entomology, National Taiwan University, Taipei 10016, Taiwan
*
Author to whom correspondence should be addressed.
Micromachines 2019, 10(12), 837; https://doi.org/10.3390/mi10120837
Submission received: 12 November 2019 / Revised: 25 November 2019 / Accepted: 28 November 2019 / Published: 30 November 2019
(This article belongs to the Special Issue Next Generation Paper-based Devices for Analytical Applications)
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Abstract

Paper-based technologies have been drawing increasing attentions in the biosensor field due to their economical, ecofriendly, and easy-to-fabricate features. In this paper, we present a time-delay valve mechanism to automate a series of procedures for conducting competitive enzyme-linked immunosorbent assay (ELISA) on a paper-based device. The mechanism employs a controllable time-delay valve, which has surfactants to dissolve the hydrophobic barriers, in a fluid pathway. The valves can regulate the liquid and sequentially deliver the sample flow for automating ELISA procedures in microchannels. Competitive ELISA is achieved in a single step once the sample, or small molecule pesticide (e.g., Imidacloprid), is applied onto the paper-based device with a comparable sensitivity to plate-based competitive ELISA. The results further demonstrate the appositeness of using paper-based devices with the valve designs for on-the-go ELISA detection in agriculture and biomedical applications.
Keywords: microfluidic valve; competitive enzyme-linked immunosorbent assay (ELISA); pesticide residue microfluidic valve; competitive enzyme-linked immunosorbent assay (ELISA); pesticide residue

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MDPI and ACS Style

Lai, Y.-T.; Tsai, C.-H.; Hsu, J.-C.; Lu, Y.-W. Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA. Micromachines 2019, 10, 837. https://doi.org/10.3390/mi10120837

AMA Style

Lai Y-T, Tsai C-H, Hsu J-C, Lu Y-W. Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA. Micromachines. 2019; 10(12):837. https://doi.org/10.3390/mi10120837

Chicago/Turabian Style

Lai, Yu-Ting, Chia-Hsin Tsai, Ju-Chun Hsu, and Yen-Wen Lu. 2019. "Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA" Micromachines 10, no. 12: 837. https://doi.org/10.3390/mi10120837

APA Style

Lai, Y.-T., Tsai, C.-H., Hsu, J.-C., & Lu, Y.-W. (2019). Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA. Micromachines, 10(12), 837. https://doi.org/10.3390/mi10120837

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