Functional Properties of Enriched Curd with Collagen and Plant Phytochemicals for Athletes and Physiological Benefits: Evidence Data from Preclinical Trials In Vivo

Zharykbasova, Klara; Kakimov, Aitbek; Zharykbasov, Yerlan; Kakimova, Zhainagul; Guldana, Raimkhanova; Zhanna, Kozykenova; Galiya, Beisembayeva; Baigazinov, Zhanat; Kovács, Tibor; Shahrokhi, Amin

doi:10.3390/nu17213373

This is an early access version, the complete PDF, HTML, and XML versions will be available soon.

Open AccessArticle

Functional Properties of Enriched Curd with Collagen and Plant Phytochemicals for Athletes and Physiological Benefits: Evidence Data from Preclinical Trials In Vivo

by

Klara Zharykbasova

^1,*,

Aitbek Kakimov

²,

Yerlan Zharykbasov

³,

Zhainagul Kakimova

³,

Raimkhanova Guldana

³,

Kozykenova Zhanna

⁴,

Beisembayeva Galiya

³,

Zhanat Baigazinov

^5,6

,

Tibor Kovács

^6,*

and

Amin Shahrokhi

^6,*

¹

Department of Applied Biology, Alikhan Bokeikhan University, Semey 070000, Kazakhstan

²

Department of Technological Equipment, Shakarim University, Semey 071412, Kazakhstan

³

Department of Biotechnology, Shakarim University, Semey 071412, Kazakhstan

⁴

Department of Physiological Disciplines Named After T.A. Nazarova, NCJSC, Semey Medical University, Semey 070000, Kazakhstan

⁵

JSC “Park of Nuclear Technologies”, Kurchatov 071100, Kazakhstan

⁶

Research Centre for Biochemical, Environmental and Chemical Engineering, University of Pannonia, 8200 Veszprem, Hungary

^*

Authors to whom correspondence should be addressed.

Nutrients 2025, 17(21), 3373; https://doi.org/10.3390/nu17213373 (registering DOI)

Submission received: 24 September 2025 / Revised: 20 October 2025 / Accepted: 22 October 2025 / Published: 27 October 2025

(This article belongs to the Section Sports Nutrition)

Download Versions Notes

Abstract

Background/Objectives: The aim of this study was to establish the multifactorial physiological effect of a functional curd product enriched with collagen-containing concentrate and phytochemical extracts of various natures, under conditions of in vivo experiment. Methods: Biomarkers, such as antioxidant activity (glutathione peroxidase, glutathione reductase, MDA), immune response (IgA, IgG, IgM, IL-6, TNF-α), and purine metabolism (uric acid, xanthine oxidase, 5′-nucleotidase) were selected for evaluation and their influence change. The model was white outbred rats (n = 45), randomly distributed into three groups: control (basic product), experimental group 1 (supplements of collagen-containing concentrate and extract of the composition of sea buckthorn and rosehips), and experimental group 2 (supplements of collagen-containing concentrate and extract of the composition of yarrow and sage). Results: In both experimental groups, a reliable increase in the enzymatic activity of the antioxidant system, a decrease in lipid peroxidation and the level of proinflammatory cytokines, an increase in immunoglobulins, and activation of 5′-nucleotidase were observed. The most pronounced effects were observed with the introduction of a curd product containing collagen-containing concentrate and sea buckthorn and rosehip extract. Conclusions: The scientific novelty of the study lies in the first comprehensive in vivo evaluation of the combined enrichment of a dairy product with collagen and plant extracts for a set of biomarkers. The data obtained confirm the physiological activity and functional properties of the developed product, which can be considered as a promising means of specialized and sports nutrition with proven biological action.

Keywords: functional foods; collagen-containing concentrate; cottage cheese product; collagen-containing concentrate; sports nutrition; phytochemical extracts; in vivo experiment

Share and Cite

MDPI and ACS Style

Zharykbasova, K.; Kakimov, A.; Zharykbasov, Y.; Kakimova, Z.; Guldana, R.; Zhanna, K.; Galiya, B.; Baigazinov, Z.; Kovács, T.; Shahrokhi, A. Functional Properties of Enriched Curd with Collagen and Plant Phytochemicals for Athletes and Physiological Benefits: Evidence Data from Preclinical Trials In Vivo. Nutrients 2025, 17, 3373. https://doi.org/10.3390/nu17213373

AMA Style

Zharykbasova K, Kakimov A, Zharykbasov Y, Kakimova Z, Guldana R, Zhanna K, Galiya B, Baigazinov Z, Kovács T, Shahrokhi A. Functional Properties of Enriched Curd with Collagen and Plant Phytochemicals for Athletes and Physiological Benefits: Evidence Data from Preclinical Trials In Vivo. Nutrients. 2025; 17(21):3373. https://doi.org/10.3390/nu17213373

Chicago/Turabian Style

Zharykbasova, Klara, Aitbek Kakimov, Yerlan Zharykbasov, Zhainagul Kakimova, Raimkhanova Guldana, Kozykenova Zhanna, Beisembayeva Galiya, Zhanat Baigazinov, Tibor Kovács, and Amin Shahrokhi. 2025. "Functional Properties of Enriched Curd with Collagen and Plant Phytochemicals for Athletes and Physiological Benefits: Evidence Data from Preclinical Trials In Vivo" Nutrients 17, no. 21: 3373. https://doi.org/10.3390/nu17213373

APA Style

Zharykbasova, K., Kakimov, A., Zharykbasov, Y., Kakimova, Z., Guldana, R., Zhanna, K., Galiya, B., Baigazinov, Z., Kovács, T., & Shahrokhi, A. (2025). Functional Properties of Enriched Curd with Collagen and Plant Phytochemicals for Athletes and Physiological Benefits: Evidence Data from Preclinical Trials In Vivo. Nutrients, 17(21), 3373. https://doi.org/10.3390/nu17213373

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Article metric data becomes available approximately 24 hours after publication online.

Article Menu

Functional Properties of Enriched Curd with Collagen and Plant Phytochemicals for Athletes and Physiological Benefits: Evidence Data from Preclinical Trials In Vivo

Abstract

Share and Cite

Article Metrics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI