Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Cell Culture
2.3. Measurement of Cytotoxicity
2.4. UVA Irradiation Procedure
2.5. Protective Effects of Puerarin on UVA-Induced Oxidative Stress
2.6. Intracellular ROS Content Measurement
2.7. Measurement of Intracellular Total Antioxidant Capacity (ABTS Method), MDA Content, and Antioxidant Enzyme Activity
2.8. ELISA
2.9. RT-PCR
2.10. Experiments on Animals
2.10.1. Experimental Animals and Groups
2.10.2. Moisturizing Experiment
2.10.3. Skin Tissue Total Antioxidant Capacity and CAT Activity Measurement
2.10.4. Hematoxylin-Eosin (HE) Dye
2.11. Statistical Analysis
3. Results
3.1. Effects of Puerarin on HSF
3.2. Effects of Puerarin on Intracellular Antioxidant Capacity
3.3. Effects of Puerarin on UVA-Induced Cell Senescence
3.4. Protective Mechanism of Puerarin against Oxidative Stress in Mice
3.4.1. Blood Routine Test Results
3.4.2. Moisturizing Experiment
3.4.3. Total Antioxidant Capacity and CAT Enzyme Activity in Mice Tissue
3.4.4. Skin Histological Observation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Blank Group | Model Group | VC Group | Puerarin Group | |
---|---|---|---|---|
WBC (109/L) | 7.53 ± 1.11 | 7.55 ± 0.89 | 4.17 ± 0.42 | 7.5 ± 2.26 |
RBC (1012/L) | 10.1 ± 1.18 | 9.85 ± 0.42 | 9.6 ± 0.26 | 10.93 ± 0.97 |
Hb (g/L) | 162 ± 8.54 | 155.25 ± 9.91 | 158.67 ± 7.77 | 175.5 ± 16.11 |
HCT | 0.50 ± 0.03 | 0.47 ± 0.04 | 0.48 ± 0.03 | 0.54 ± 0.05 |
MCV (fL) | 52.33 ± 1.53 | 47.75 ± 2.36 | 51.5 ± 1.53 | 49.25 ± 1.71 |
MCH (Pg) | 16.33 ± 1.15 | 15.75 ± 0.5 | 16.67 ± 0.58 | 16 ± 0.82 |
MCHC (g/L) | 323.67 ± 2.08 | 328.25 ± 10.01 | 328 ± 8.72 | 326 ± 2.94 |
PLT (109/L) | 1039.33 ± 218.48 | 744.25 ± 112 | 689.33 ± 165.03 | 835.75 ± 235.36 |
N# (109/L) | 2.33 ± 0.97 | 2.83 ± 1.54 | 1.55 ± 0.36 | 2.8 ± 1.67 |
L# (109/L) | 5.75 ± 0.85 | 5.65 ± 0.05 | 2.45 ± 0.4 | 4.77 ± 1.78 |
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Gene | Direction | Primer Pair Sequence (5′→3′) |
---|---|---|
β-actin | F | TGGCACCCAGCACAATGAA |
R | CTAAGTCATAGTCCGCCTAGAAGCA | |
Nrf2 | F | CAACTCAGCACCTTGTATC |
R | TTCTTAGTATCTGGCTTCTT | |
KEAP1 | F | GGAGGCGGAGCCCGA |
R | GATGCCCTCAATGGACACCA | |
HO-1 | F | CAAGCGCTATGTTCAGCGAC |
R | GCTTGAACTTGGTGGCACTG | |
NQO1 | F | CAGCCAATCAGCGTTCGGTA |
R | CTTCATGGCGTAGTTGAATGATGTC | |
GCLC | F | CAGTCAAGGACCGGCACAAG |
R | CAAGAACATCGCCTCCATTCAG | |
ERK | F | TGTTCCCAAATGCTGACTCCAA |
R | TCGGGTCGTAATACTGCTCCAGATA | |
JNK | F | CTGTGTGGAATCAAGCACCTTCA |
R | CTGGCCAGACCGAAGTCAAGA | |
P38 | F | TTAACAGGATGCCAAGCCATGA |
R | GGCACCAATAAATACATTCGCAAAG |
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Mo, Q.; Li, S.; You, S.; Wang, D.; Zhang, J.; Li, M.; Wang, C. Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways. Nutrients 2022, 14, 4724. https://doi.org/10.3390/nu14224724
Mo Q, Li S, You S, Wang D, Zhang J, Li M, Wang C. Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways. Nutrients. 2022; 14(22):4724. https://doi.org/10.3390/nu14224724
Chicago/Turabian StyleMo, Qiuting, Shuping Li, Shiquan You, Dongdong Wang, Jiachan Zhang, Meng Li, and Changtao Wang. 2022. "Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways" Nutrients 14, no. 22: 4724. https://doi.org/10.3390/nu14224724