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A Fish-Derived Protein Hydrolysate Induces Postprandial Aminoacidaemia and Skeletal Muscle Anabolism in an In Vitro Cell Model Using Ex Vivo Human Serum

1
Department of Physical Education and Sport Sciences, Faculty of Education and Health Sciences, University of Limerick, V94 T9PX Limerick, Ireland
2
School of Health and Human Performance, Dublin City University, D09 V209 Dublin, Ireland
3
Health Research Institute, University of Limerick, V94 T9PX Limerick, Ireland
4
Department of Biological Sciences, University of Limerick, V94 T9PX Limerick, Ireland
*
Author to whom correspondence should be addressed.
Academic Editor: Sara Salucci
Nutrients 2021, 13(2), 647; https://doi.org/10.3390/nu13020647
Received: 26 January 2021 / Revised: 9 February 2021 / Accepted: 12 February 2021 / Published: 17 February 2021
(This article belongs to the Special Issue Nutrition and Regulation of Muscle Protein Synthesis)
Fish-derived proteins, particularly fish protein hydrolysates (FPH), offer potential as high-quality sources of dietary protein, whilst enhancing economic and environmental sustainability. This study investigated the impact of a blue whiting-derived protein hydrolysate (BWPH) on aminoacidaemia in vivo and skeletal muscle anabolism in vitro compared with whey protein isolate (WPI) and an isonitrogenous, non-essential amino acid (NEAA) control (0.33 g·kg−1·body mass−1) in an ex vivo, in vitro experimental design. Blood was obtained from seven healthy older adults (two males, five females; age: 72 ± 5 years, body mass index: 24.9 ± 1.6 kg·m2) in three separate trials in a randomised, counterbalanced, double-blind design. C2C12 myotubes were treated with ex vivo human serum-conditioned media (20%) for 4 h. Anabolic signalling (phosphorylation of mTOR, p70S6K, and 4E-BP1) and puromycin incorporation were determined by immunoblotting. Although BWPH and WPI both induced postprandial essential aminoacidaemia in older adults above the NEAA control, peak and area under the curve (AUC) leucine and essential amino acids were more pronounced following WPI ingestion. Insulin was elevated above baseline in WPI and BWPH only, a finding reinforced by higher peak and AUC values compared with NEAA. Muscle protein synthesis, as measured by puromycin incorporation, was greater after incubation with WPI-fed serum compared with fasted serum (P = 0.042), and delta change was greater in WPI (P = 0.028) and BWPH (P = 0.030) compared with NEAA. Myotube hypertrophy was greater in WPI and BWPH compared with NEAA (both P = 0.045), but was similar between bioactive conditions (P = 0.853). Taken together, these preliminary findings demonstrate the anabolic potential of BWPH in vivo and ex vivo, thus providing justification for larger studies in older adults using gold-standard measures of acute and chronic MPS in vivo. View Full-Text
Keywords: ageing; blue whiting; hypertrophy; leucine; mTORC1; muscle protein synthesis ageing; blue whiting; hypertrophy; leucine; mTORC1; muscle protein synthesis
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MDPI and ACS Style

Lees, M.J.; Nolan, D.; Amigo-Benavent, M.; Raleigh, C.J.; Khatib, N.; Harnedy-Rothwell, P.; FitzGerald, R.J.; Egan, B.; Carson, B.P. A Fish-Derived Protein Hydrolysate Induces Postprandial Aminoacidaemia and Skeletal Muscle Anabolism in an In Vitro Cell Model Using Ex Vivo Human Serum. Nutrients 2021, 13, 647. https://doi.org/10.3390/nu13020647

AMA Style

Lees MJ, Nolan D, Amigo-Benavent M, Raleigh CJ, Khatib N, Harnedy-Rothwell P, FitzGerald RJ, Egan B, Carson BP. A Fish-Derived Protein Hydrolysate Induces Postprandial Aminoacidaemia and Skeletal Muscle Anabolism in an In Vitro Cell Model Using Ex Vivo Human Serum. Nutrients. 2021; 13(2):647. https://doi.org/10.3390/nu13020647

Chicago/Turabian Style

Lees, Matthew J., David Nolan, Miryam Amigo-Benavent, Conor J. Raleigh, Neda Khatib, Pádraigín Harnedy-Rothwell, Richard J. FitzGerald, Brendan Egan, and Brian P. Carson 2021. "A Fish-Derived Protein Hydrolysate Induces Postprandial Aminoacidaemia and Skeletal Muscle Anabolism in an In Vitro Cell Model Using Ex Vivo Human Serum" Nutrients 13, no. 2: 647. https://doi.org/10.3390/nu13020647

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