Next Article in Journal
Use of Remote Monitoring by E-mail for Long-Term Management of the Classic Ketogenic Diet
Next Article in Special Issue
Effects of 3-Month Astaxanthin Supplementation on Cardiac Function in Heart Failure Patients with Left Ventricular Systolic Dysfunction-A Pilot Study
Previous Article in Journal
Effect of Gluten-Free Diet on Gut Microbiota Composition in Patients with Celiac Disease and Non-Celiac Gluten/Wheat Sensitivity
Previous Article in Special Issue
Are Supplements Safe? Effects of Gallic and Ferulic Acids on In Vitro Cell Models
Article

Blueberry Counteracts BV-2 Microglia Morphological and Functional Switch after LPS Challenge

1
Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161 Rome, Italy
2
Department of Biochemical Sciences, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
3
Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy
*
Author to whom correspondence should be addressed.
Nutrients 2020, 12(6), 1830; https://doi.org/10.3390/nu12061830
Received: 29 April 2020 / Revised: 4 June 2020 / Accepted: 16 June 2020 / Published: 19 June 2020
(This article belongs to the Special Issue Dietary Antioxidants for Human Health)
Microglia, the innate immune cells of the CNS, respond to brain injury by activating and modifying their morphology. Our study arises from the great interest that has been focused on blueberry (BB) for the antioxidant and pharmacological properties displayed by its components. We analyzed the influence of hydroalcoholic BB extract in resting or lipopolysaccharide (LPS)-stimulated microglia BV-2 cells. BB exerted a protective effect against LPS-induced cytotoxicity, as indicated by cell viability. BB was also able to influence the actin cytoskeleton organization, to recover the control phenotype after LPS insult, and also to reduce LPS-driven migration. We evaluated the activity of Rho and Rac1 GTPases, which regulate both actin cytoskeletal organization and migratory capacity. LPS caused an increase in Rac1 activity, which was counteracted by BB extract. Furthermore, we demonstrated that, in the presence of BB, mRNA expression of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α decreased, as did the immunofluorescence signal of iNOS, whereas that of Arg-1 was increased. Taken together, our results show that, during the inflammatory response, BB extract shifts the M1 polarization towards the M2 phenotype through an actin cytoskeletal rearrangement. Based on that, we might consider BB as a nutraceutical with anti-inflammatory activities. View Full-Text
Keywords: blueberry; BV-2 cells; actin cytoskeleton; cell migration; Rho; Rac1; M1/M2 phenotypes; inflammatory cytokines blueberry; BV-2 cells; actin cytoskeleton; cell migration; Rho; Rac1; M1/M2 phenotypes; inflammatory cytokines
Show Figures

Figure 1

MDPI and ACS Style

De Caris, M.G.; Grieco, M.; Maggi, E.; Francioso, A.; Armeli, F.; Mosca, L.; Pinto, A.; D’Erme, M.; Mancini, P.; Businaro, R. Blueberry Counteracts BV-2 Microglia Morphological and Functional Switch after LPS Challenge. Nutrients 2020, 12, 1830. https://doi.org/10.3390/nu12061830

AMA Style

De Caris MG, Grieco M, Maggi E, Francioso A, Armeli F, Mosca L, Pinto A, D’Erme M, Mancini P, Businaro R. Blueberry Counteracts BV-2 Microglia Morphological and Functional Switch after LPS Challenge. Nutrients. 2020; 12(6):1830. https://doi.org/10.3390/nu12061830

Chicago/Turabian Style

De Caris, Maria G., Maddalena Grieco, Elisa Maggi, Antonio Francioso, Federica Armeli, Luciana Mosca, Alessandro Pinto, Maria D’Erme, Patrizia Mancini, and Rita Businaro. 2020. "Blueberry Counteracts BV-2 Microglia Morphological and Functional Switch after LPS Challenge" Nutrients 12, no. 6: 1830. https://doi.org/10.3390/nu12061830

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop