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Comment published on 28 February 2022, see Viruses 2022, 14(3), 495.
Article

Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments

by 1,2, 1, 1,2, 1, 3,4,5, 3,4,5 and 1,2,*
1
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
2
College of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China
3
Key Laboratory for Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
4
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
5
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266273, China
*
Author to whom correspondence should be addressed.
Academic Editor: Evelien Adriaenssens
Viruses 2022, 14(1), 108; https://doi.org/10.3390/v14010108
Received: 19 November 2021 / Revised: 25 December 2021 / Accepted: 6 January 2022 / Published: 8 January 2022
(This article belongs to the Section Bacterial Viruses)
Viruses are far more abundant than cellular microorganisms in the marine ecosystem. However, very few viruses have so far been isolated from marine sediments, especially hydrothermal vent sediments, hindering the understanding of the biology and ecological functions of these tiny organisms. Here, we report the isolation and characterization of a temperate bacteriophage, named PVJ1, which infects Psychrobacillus from a hydrothermal vent field in Okinawa Trough. PVJ1 belongs to the Myoviridae family of the order Caudovirales. The tailed phage possesses a 53,187 bp linear dsDNA genome, with 84 ORFs encoding structural proteins, genome replication, host lysis, etc. in a modular pattern. The phage genome is integrated into the host chromosome near the 3′-end of deoD, a gene encoding purine nucleoside phosphorylase (PNP). The phage integration does not appear to disrupt the function of PNP. The phage DNA is packaged by the headful mechanism. Release of PVJ1 from the host cell was drastically enhanced by treatment with mitomycin C. Phages encoding an MCP sharing significant similarity (≥70% identical amino acids) with that of PVJ1 are widespread in diverse environments, including marine and freshwater sediments, soils, artificial ecosystems, and animal intestines, and primarily infect Firmicutes. These results are valuable to the understanding of the lifestyle and host interactions of bacterial viruses at the bottom of the ocean. View Full-Text
Keywords: Psychrobacillus; bacteriophage; marine sediments; Myoviridae Psychrobacillus; bacteriophage; marine sediments; Myoviridae
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MDPI and ACS Style

Liu, W.; Zheng, X.; Dai, X.; Zhang, Z.; Zhang, W.; Xiao, T.; Huang, L. Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments. Viruses 2022, 14, 108. https://doi.org/10.3390/v14010108

AMA Style

Liu W, Zheng X, Dai X, Zhang Z, Zhang W, Xiao T, Huang L. Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments. Viruses. 2022; 14(1):108. https://doi.org/10.3390/v14010108

Chicago/Turabian Style

Liu, Wang, Xiaowei Zheng, Xin Dai, Zhenfeng Zhang, Wenyan Zhang, Tian Xiao, and Li Huang. 2022. "Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments" Viruses 14, no. 1: 108. https://doi.org/10.3390/v14010108

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