Next Article in Journal
Citrus Tristeza Virus Genotype Detection Using High-Throughput Sequencing
Next Article in Special Issue
Multiscale Electron Microscopy for the Study of Viral Replication Organelles
Previous Article in Journal
Induction of Brain Tumors by the Archetype Strain of Human Neurotropic JCPyV in a Transgenic Mouse Model
Previous Article in Special Issue
Single-Molecule Super-Resolution Imaging of T-Cell Plasma Membrane CD4 Redistribution upon HIV-1 Binding
Review

Nuclear Import of Adeno-Associated Viruses Imaged by High-Speed Single-Molecule Microscopy

Department of Biology, Temple University, 1900 N. 12th Street, Philadelphia, PA 19122, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Cristina Risco
Viruses 2021, 13(2), 167; https://doi.org/10.3390/v13020167
Received: 1 December 2020 / Revised: 21 January 2021 / Accepted: 21 January 2021 / Published: 22 January 2021
Understanding the detailed nuclear import kinetics of adeno-associated virus (AAV) through the nuclear pore complex (NPC) is essential for the application of AAV capsids as a nuclear delivery instrument as well as a target for drug development. However, a comprehensive understanding of AAV transport through the sub-micrometer NPCs in live cells calls for new techniques that can conquer the limitations of conventional fluorescence microscopy and electron microscopy. With recent technical advances in single-molecule fluorescence microscopy, we are now able to image the entire nuclear import process of AAV particles and also quantify the transport dynamics of viral particles through the NPCs in live human cells. In this review, we initially evaluate the necessity of single-molecule live-cell microscopy in the study of nuclear import for AAV particles. Then, we detail the application of high-speed single-point edge-excitation sub-diffraction (SPEED) microscopy in tracking the entire process of nuclear import for AAV particles. Finally, we summarize the major findings for AAV nuclear import by using SPEED microscopy. View Full-Text
Keywords: nucleocytoplasmic transport; super-resolution microscopy; virus-host Interactions nucleocytoplasmic transport; super-resolution microscopy; virus-host Interactions
Show Figures

Figure 1

MDPI and ACS Style

Junod, S.L.; Saredy, J.; Yang, W. Nuclear Import of Adeno-Associated Viruses Imaged by High-Speed Single-Molecule Microscopy. Viruses 2021, 13, 167. https://doi.org/10.3390/v13020167

AMA Style

Junod SL, Saredy J, Yang W. Nuclear Import of Adeno-Associated Viruses Imaged by High-Speed Single-Molecule Microscopy. Viruses. 2021; 13(2):167. https://doi.org/10.3390/v13020167

Chicago/Turabian Style

Junod, Samuel L., Jason Saredy, and Weidong Yang. 2021. "Nuclear Import of Adeno-Associated Viruses Imaged by High-Speed Single-Molecule Microscopy" Viruses 13, no. 2: 167. https://doi.org/10.3390/v13020167

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop