Adenovirus 14p1 Immunopathogenesis during Lung Infection in the Syrian Hamster
Abstract
:1. Introduction
2. Materials and Methods
2.1. Viruses
2.2. Animals
2.3. Ethics Statement
2.4. Viral Infection of Animals
2.5. Histopathology of Lungs
2.6. Immunohistochemistry
2.7. Recovery of Immune Cells from Bronchoalveolar Lavage Fluid
2.8. Differential Cell Counts on BALF Cells
2.9. Quantitation of Ad14 Genomes in Hamster Lungs
2.10. Cytokine Gene Expression
2.11. Statistical Analysis
3. Results
3.1. Ad14p1 Induces Severe Inflammation in the Lungs of Syrian Hamsters
3.2. Ad14 and Ad14p1 Induce a Mixed Leukocyte Immune Response
3.3. Ad14 and Ad14p1 Replicate in Syrian Hamsters
3.4. Ad14 and Ad14p1 Infections Are Associated with Different Cytokine/Chemokine Profiles
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Radke, J.R.; Covert, H.J.; Bauer, F.; Ananthanarayanan, V.; Cook, J.L. Adenovirus 14p1 Immunopathogenesis during Lung Infection in the Syrian Hamster. Viruses 2020, 12, 595. https://doi.org/10.3390/v12060595
Radke JR, Covert HJ, Bauer F, Ananthanarayanan V, Cook JL. Adenovirus 14p1 Immunopathogenesis during Lung Infection in the Syrian Hamster. Viruses. 2020; 12(6):595. https://doi.org/10.3390/v12060595
Chicago/Turabian StyleRadke, Jay R., Hunter J. Covert, Fredrick Bauer, Vijayalakshmi Ananthanarayanan, and James L. Cook. 2020. "Adenovirus 14p1 Immunopathogenesis during Lung Infection in the Syrian Hamster" Viruses 12, no. 6: 595. https://doi.org/10.3390/v12060595