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Article

Discovery of Three Toxic Proteins of Klebsiella Phage fHe-Kpn01

1
Department of Bacteriology and Immunology, Medicum, Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00290 Helsinki, Finland
2
Laboratory of Microbiology, Wageningen University and Research, 6708 WE Wageningen, The Netherlands
3
Division of Clinical Microbiology, HUSLAB, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, Finland
4
Department of Microbiology, Faculty of Agriculture and Forestry, University of Helsinki, 00790 Helsinki, Finland
*
Author to whom correspondence should be addressed.
Present address: Department of Chemical Biology & Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CG Utrecht, The Netherlands.
Viruses 2020, 12(5), 544; https://doi.org/10.3390/v12050544
Received: 3 May 2020 / Revised: 12 May 2020 / Accepted: 13 May 2020 / Published: 15 May 2020
(This article belongs to the Section Bacterial Viruses)
The lytic phage, fHe-Kpn01 was isolated from sewage water using an extended-spectrum beta-lactamase-producing strain of Klebsiella pneumoniae as a host. The genome is 43,329 bp in size and contains direct terminal repeats of 222 bp. The genome contains 56 predicted genes, of which proteomics analysis detected 29 different proteins in purified phage particles. Comparison of fHe-Kpn01 to other phages, both morphologically and genetically, indicated that the phage belongs to the family Podoviridae and genus Drulisvirus. Because fHe-Kpn01 is strictly lytic and does not carry any known resistance or virulence genes, it is suitable for phage therapy. It has, however, a narrow host range since it infected only three of the 72 tested K. pneumoniae strains, two of which were of capsule type KL62. After annotation of the predicted genes based on the similarity to genes of known function and proteomics results on the virion-associated proteins, 22 gene products remained annotated as hypothetical proteins of unknown function (HPUF). These fHe-Kpn01 HPUFs were screened for their toxicity in Escherichia coli. Three of the HPUFs, encoded by the genes g10, g22, and g38, were confirmed to be toxic. View Full-Text
Keywords: bacteriophage; Podoviridae; Drulisvirus; hypothetical proteins of unknown function; toxic proteins; antibiotic resistance; antibiotics; Klebsiella pneumoniae; capsule type bacteriophage; Podoviridae; Drulisvirus; hypothetical proteins of unknown function; toxic proteins; antibiotic resistance; antibiotics; Klebsiella pneumoniae; capsule type
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MDPI and ACS Style

Spruit, C.M.; Wicklund, A.; Wan, X.; Skurnik, M.; Pajunen, M.I. Discovery of Three Toxic Proteins of Klebsiella Phage fHe-Kpn01. Viruses 2020, 12, 544. https://doi.org/10.3390/v12050544

AMA Style

Spruit CM, Wicklund A, Wan X, Skurnik M, Pajunen MI. Discovery of Three Toxic Proteins of Klebsiella Phage fHe-Kpn01. Viruses. 2020; 12(5):544. https://doi.org/10.3390/v12050544

Chicago/Turabian Style

Spruit, Cindy M., Anu Wicklund, Xing Wan, Mikael Skurnik, and Maria I. Pajunen 2020. "Discovery of Three Toxic Proteins of Klebsiella Phage fHe-Kpn01" Viruses 12, no. 5: 544. https://doi.org/10.3390/v12050544

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