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Open AccessArticle

Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study

1
Aerobiological Laboratory, Antimicrobial Agents Laboratory, State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia
2
Astrakhan State Medical University, 414000 Astrakhan, Russia
3
N.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia
4
Lomonosov Moscow State University, 119991 Moscow, Russia
5
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences. 33, bld. 2 Leninsky Ave., 119071 Moscow, Russia
6
Center for Strategic Planning of the Ministry of Health of the Russian Federation, 119435 Moscow, Russia
7
Infectiology Department, I. M. Sechenov First Moscow State Medical University, 119146 Moscow, Russia
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G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, 125212 Moscow, Russia
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Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany
10
Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, 30625 Hannover, Germany
*
Authors to whom correspondence should be addressed.
Contributed equally to this article.
Equally managed the research.
Viruses 2020, 12(5), 545; https://doi.org/10.3390/v12050545
Received: 1 April 2020 / Revised: 2 May 2020 / Accepted: 11 May 2020 / Published: 15 May 2020
(This article belongs to the Special Issue Bacteriophages and Biofilms)
Surfaces of implanted medical devices are highly susceptible to biofilm formation. Bacteria in biofilms are embedded in a self-produced extracellular matrix that inhibits the penetration of antibiotics and significantly contributes to the mechanical stability of the colonizing community which leads to an increase in morbidity and mortality rate in clinical settings. Therefore, new antibiofilm approaches and substances are urgently needed. In this paper, we test the efficacy of a broad-range recombinant endolysin of the coliphage LysECD7 against forming and mature biofilms. We used a strong biofilm producer—Klebsiella pneumoniae Ts 141-14 clinical isolate. In vitro investigation of the antibacterial activity was performed using the standard biofilm assay in microtiter plates. We optimized the implantable diffusion chamber approach in order to reach strong biofilm formation in vivo avoiding severe consequences of the pathogen for the animals and to obtain a well-reproducible model of implant-associated infection. Endolysin LysECD7 significantly reduced the biofilm formation and was capable of degrading the preformed biofilm in vitro. The animal trials on the preformed biofilms confirmed these results. Overall, our results show that LysECD7 is a promising substance against clinically relevant biofilms. View Full-Text
Keywords: endolysin; biofilm degradation; drug-resistant bacteria; implant-associated infection model; animal trial endolysin; biofilm degradation; drug-resistant bacteria; implant-associated infection model; animal trial
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MDPI and ACS Style

Fursov, M.V.; Abdrakhmanova, R.O.; Antonova, N.P.; Vasina, D.V.; Kolchanova, A.D.; Bashkina, O.A.; Rubalsky, O.V.; Samotrueva, M.A.; Potapov, V.D.; Makarov, V.V.; Yudin, S.M.; Gintsburg, A.L.; Tkachuk, A.P.; Gushchin, V.A.; Rubalskii, E.O. Antibiofilm Activity of a Broad-Range Recombinant Endolysin LysECD7: In Vitro and In Vivo Study. Viruses 2020, 12, 545.

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