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Viruses 2019, 11(1), 81; https://doi.org/10.3390/v11010081

Detection and Characterization of Cucumis melo Cryptic Virus, Cucumis melo Amalgavirus 1, and Melon Necrotic Spot Virus in Cucumis melo

1
State Key Laboratory for Biology of Plant Disease and Insect Pest, Institute of Plant Protection, Academy of Agricultural Sciences, Beijing 100193, China
2
National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400715, China
3
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China
*
Author to whom correspondence should be addressed.
Received: 6 November 2018 / Revised: 6 January 2019 / Accepted: 11 January 2019 / Published: 18 January 2019
(This article belongs to the Special Issue Plant Virus Ecology and Biodiversity)
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Abstract

Three RNA viruses—Cucumis melo cryptic virus (CmCV), Cucumis melo amalgavirus 1 (CmAV1), and melon necrotic spot virus (MNSV)—were identified from a melon (Cucumis melo) transcriptome dataset. CmCV has two dsRNA genome segments; dsRNA-1 is 1592 bp in size, containing a conserved RNA-dependent RNA polymerase (RdRp), and dsRNA-2 is 1715 bp in size, and encodes a coat protein (CP). The sequence alignment and phylogenetic analyses of the CmCV RdRp and CP indicated CmCV clusters with approved or putative deltapartitiviruses in well-supported monophyletic clade. The RdRp of CmCV shared an amino acid sequence identity of 60.7% with the closest RdRp of beet cryptic virus 3, and is <57% identical to other partitiviruses. CmAV1 is a nonsegmented dsRNA virus with a genome of 3424 bp, including two partially overlapping open reading frames (ORFs) encoding a putative CP and RdRp. The sequence alignment and phylogenetic analyses of CmAV1 RdRp revealed that it belongs to the genus Amalgavirus in the family Amalgaviridae. The RdRp of CmAV1 shares 57.7% of its amino acid sequence identity with the most closely related RdRp of Phalaenopsis equestris amalgavirus 1, and is <47% identical to the other reported amalgaviruses. These analyses suggest that CmCV and CmAV1 are novel species in the genera Amalgavirus and Deltapartitivirus, respectively. These findings enrich our understanding of new plant dsRNA virus species. View Full-Text
Keywords: melon (Cucumis melo); transcriptome sequencing; dsRNA virus; Amalgavirus; Deltapartitivirus melon (Cucumis melo); transcriptome sequencing; dsRNA virus; Amalgavirus; Deltapartitivirus
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Zhan, B.; Cao, M.; Wang, K.; Wang, X.; Zhou, X. Detection and Characterization of Cucumis melo Cryptic Virus, Cucumis melo Amalgavirus 1, and Melon Necrotic Spot Virus in Cucumis melo. Viruses 2019, 11, 81.

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