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In Vitro Physico-Chemical Characterization and Standardized In Vivo Evaluation of Biocompatibility of a New Synthetic Membrane for Guided Bone Regeneration

1
Graduate Program in Dentistry, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24.020-140, Brazil
2
Oral Surgery Department, Dentistry School, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24.020-140, Brazil
3
Oral Diagnosis Department, Dentistry School, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24.020-140, Brazil
4
Bioengineering Program, National Institute of Metrology, Quality and Technology, Duque de Caxias, Rio de Janeiro 24.020-140, Brizil
*
Author to whom correspondence should be addressed.
Materials 2019, 12(7), 1186; https://doi.org/10.3390/ma12071186
Received: 25 January 2019 / Revised: 8 April 2019 / Accepted: 9 April 2019 / Published: 11 April 2019
(This article belongs to the Special Issue Current and Future Trends in Dental Materials)
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Abstract

This study’s aim was to evaluate the biocompatibility and bioabsorption of a new membrane for guided bone regeneration (polylactic-co-glycolic acid associated with hydroxyapatite and β-tricalcium phosphate) with three thicknesses (200, 500, and 700 µm) implanted in mice subcutaneously. Scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and the quantification of carbon, hydrogen and nitrogen were used to characterize the physico-chemical properties. One hundred Balb-C mice were divided into 5 experimental groups: Group 1—Sham (without implantation); Group 2—200 μm; Group 3—500 μm; Group 4—700 μm; and Group 5—Pratix®. Each group was subdivided into four experimental periods (7, 30, 60 and 90 days). Samples were collected and processed for histological and histomorphometrical evaluation. The membranes showed no moderate or severe tissue reactions during the experimental periods studied. The 500-μm membrane showed no tissue reaction during any experimental period. The 200-μm membrane began to exhibit fragmentation after 30 days, while the 500-μm and 700-µm membranes began fragmentation at 90 days. All membranes studied were biocompatible and the 500 µm membrane showed the best results for absorption and tissue reaction, indicating its potential for clinical guided bone regeneration. View Full-Text
Keywords: membranes; polymer; biocompatibility; inflammatory cells; calcium phosphate membranes; polymer; biocompatibility; inflammatory cells; calcium phosphate
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da Costa Pereira, L.; de Almeida Barros Mourão, C.F.; Neves Novellino Alves, A.T.; Figueiredo de Brito Resende, R.; Pinheiro Guedes de Uzeda, M.J.; Granjeiro, J.M.; Seabra Louro, R.; Diuana Calasans-Maia, M. In Vitro Physico-Chemical Characterization and Standardized In Vivo Evaluation of Biocompatibility of a New Synthetic Membrane for Guided Bone Regeneration. Materials 2019, 12, 1186.

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