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Open AccessArticle

Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host

by Mingwei Shao 1,2, Juying Ma 1,2, Qinglian Li 1,2 and Jianhua Ju 1,2,*
1
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China
2
University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 110039, China
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(2), 127; https://doi.org/10.3390/md17020127
Received: 1 February 2019 / Revised: 15 February 2019 / Accepted: 17 February 2019 / Published: 21 February 2019
(This article belongs to the Special Issue Deep-Sea Natural Products II)
Aborycin is a ribosomally synthesized member of the type I lasso peptide natural products. In the present study, aborycin was isolated and identified from the deep-sea-derived microbe Streptomyces sp. SCSIO ZS0098. The aborycin biosynthetic gene cluster (abo) was identified on the basis of genome sequence analyses and then heterologously expressed in Streptomyces coelicolor M1152 to effectively produce aborycin. Aborycin generated in this fashion exhibited moderate antibacterial activity against 13 Staphylococcus aureus strains from various sources with minimum inhibitory concentrations MICs = 8.0~128 µg/mL, against Enterococcus faecalis ATCC 29212 with an MIC = 8.0 µg/mL, and against Bacillus thuringiensis with MIC = 2.0 µg/mL. Additionally, aborycin displayed potent antibacterial activity (MIC = 0.5 µg/mL) against the poultry pathogen Enterococcus gallinarum 5F52C. The reported abo cluster clearly has the potential to provide a means of expanding the repertoire of anti-infective type I lasso peptides. View Full-Text
Keywords: Streptomyces sp. SCSIO ZS0098; aborycin; heterogenous expression; antibacterial activity Streptomyces sp. SCSIO ZS0098; aborycin; heterogenous expression; antibacterial activity
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Shao, M.; Ma, J.; Li, Q.; Ju, J. Identification of the Anti-Infective Aborycin Biosynthetic Gene Cluster from Deep-Sea-Derived Streptomyces sp. SCSIO ZS0098 Enables Production in a Heterologous Host. Mar. Drugs 2019, 17, 127.

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