Review Reports

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The manuscript ‘Biofunctionalisation of Metals for Medical Application: Molecular Aspects’ outlines various methods. The review links the structure to intracellular gene delivery. It is written in clear language, albeit with very long sentences.

To improve the review, it would be worth making a few minor adjustments.

- The abstract is too vague. It would be helpful to see the main message of the review and its structure.

- I would expand the title by replacing ‘metals’ with ‘materials’. Not everything you describe uses metals.

- There is a lack of statistics on the scheme. It would be good to see tables with characteristics such as particle size, charge, and transfection efficiency. Furthermore, there is a lack of a table on the aspects of material delivery, including parameters such as toxicity, disease, clinical stages, and outcomes.

- A critical analysis would also be welcome. There is a lack of information on the shortcomings of the systems and the limitations that prevent progress in the medical field.

- I would also like to see a section on design rules. This should outline the rules and restrictions that researchers face when building new systems. What requirements must they meet, and what is common to all approaches?

 

 

Author Response

please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

This is an interesting review article on the use of peptide delivery systems. I do have some comments.
1) Please contextualize more the need for these forms of therapies.
2) Look at other potential applications for gene therapy such as agriculture for example https://doi.org/10.1071/RD09211
3) You didn´t include nanoclays as another potential vector for gene therapy in table 1 for example https://doi.org/10.1016/j.jcis.2019.05.062
4) Section 4 may benefit from a table listing information such as diseases, gene treatment, and delivery mechanism with references.   

Author Response

Please see the attachment

Author Response File: Author Response.pdf