The Effect of GB1 on DSS-Induced Colitis in WT and Nlrp3-/- Mice

Zhou, Ziyi; Wang, Lixian; Liao, Ruhe; Chen, Qin; Liu, Changhui; Song, Jianping; Deng, Changsheng; Huang, Xinan

doi:10.3390/ijms26094016

Open AccessArticle

The Effect of GB1 on DSS-Induced Colitis in WT and Nlrp3^-/- Mice

by

Ziyi Zhou

^1,†,

Lixian Wang

^1,†,

Ruhe Liao

¹,

Qin Chen

¹,

Changhui Liu

²,

Jianping Song

¹,

Changsheng Deng

^1,3,* and

Xinan Huang

^1,*

¹

Artemisinin Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China

²

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510405, China

³

State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijing 100700, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2025, 26(9), 4016; https://doi.org/10.3390/ijms26094016

Submission received: 19 March 2025 / Revised: 21 April 2025 / Accepted: 23 April 2025 / Published: 24 April 2025

(This article belongs to the Section Molecular Immunology)

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Abstract

This study investigated the protective effects of Garcinia biflavonoid 1 (GB1) against dextran sulfate sodium (DSS)-induced ulcerative colitis and its underlying mechanisms. Using wild-type (WT) and NLRP3 knockout (Nlrp3^-/-) mice, we demonstrated that GB1 administration significantly ameliorated colitis symptoms, as evidenced by improved body weight, disease activity index (DAI) scores, colon length, and histological damage in WT mice. Mechanistically, GB1 downregulated pro-inflammatory mediators (IL-6, NF-κB, and CD11b) while attenuating the expression of NLRP3 inflammasome components (ASC, Caspase-1, and IL-1β). Notably, these protective effects were abolished in Nlrp3^-/- mice, confirming the essential role of NLRP3 in GB1-mediated mitigation of colitis. Furthermore, GB1 reinforced intestinal barrier integrity by preserving tight junctions, reducing permeability, and attenuating mucosal inflammation. Collectively, our findings highlight GB1 as a promising therapeutic candidate for colitis treatment, primarily through NLRP3 inflammasome suppression and intestinal barrier restoration.

Keywords: Garcinia biflavonoid 1; ulcerative colitis; NLPR3^-/- mice; inhibiting NLRP3

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MDPI and ACS Style

Zhou, Z.; Wang, L.; Liao, R.; Chen, Q.; Liu, C.; Song, J.; Deng, C.; Huang, X. The Effect of GB1 on DSS-Induced Colitis in WT and Nlrp3^-/- Mice. Int. J. Mol. Sci. 2025, 26, 4016. https://doi.org/10.3390/ijms26094016

AMA Style

Zhou Z, Wang L, Liao R, Chen Q, Liu C, Song J, Deng C, Huang X. The Effect of GB1 on DSS-Induced Colitis in WT and Nlrp3^-/- Mice. International Journal of Molecular Sciences. 2025; 26(9):4016. https://doi.org/10.3390/ijms26094016

Chicago/Turabian Style

Zhou, Ziyi, Lixian Wang, Ruhe Liao, Qin Chen, Changhui Liu, Jianping Song, Changsheng Deng, and Xinan Huang. 2025. "The Effect of GB1 on DSS-Induced Colitis in WT and Nlrp3^-/- Mice" International Journal of Molecular Sciences 26, no. 9: 4016. https://doi.org/10.3390/ijms26094016

APA Style

Zhou, Z., Wang, L., Liao, R., Chen, Q., Liu, C., Song, J., Deng, C., & Huang, X. (2025). The Effect of GB1 on DSS-Induced Colitis in WT and Nlrp3^-/- Mice. International Journal of Molecular Sciences, 26(9), 4016. https://doi.org/10.3390/ijms26094016

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The Effect of GB1 on DSS-Induced Colitis in WT and Nlrp3^-/- Mice

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