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Volume 24
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10.3390/ijms241311195
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Article

PRGF Membrane with Tailored Optical Properties Preserves the Cytoprotective Effect of Plasma Rich in Growth Factors: In Vitro Model of Retinal Pigment Epithelial Cells

by
Eduardo Anitua
1,2,*,†,
Francisco Muruzabal
1,2,†,
María de la Fuente
1,2,
Susana Del Olmo-Aguado
3,4,
Mohammad H. Alkhraisat
1,2 and
Jesús Merayo-Lloves
3,4
1
BTI—Biotechnology Institute, 01007 Vitoria, Spain
2
University Institute for Regenerative Medicine and Oral Implantology—UIRMI (UPV/EHU-Fundación Eduardo Anitua), 01007 Vitoria, Spain
3
Fundación de Investigación Oftalmológica, Instituto Oftalmológico Fernández-Vega, 33012 Oviedo, Spain
4
Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2023, 24(13), 11195; https://doi.org/10.3390/ijms241311195
Submission received: 31 May 2023 / Revised: 30 June 2023 / Accepted: 5 July 2023 / Published: 7 July 2023
(This article belongs to the Special Issue Advanced Research in Retina 2.0)
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Abstract

The present study evaluates the ability of a novel plasma rich in growth factors (PRGF) membrane with improved optical properties to reduce oxidative stress in retinal pigment epithelial cells (ARPE-19 cells) exposed to blue light. PRGF was obtained from three healthy donors and divided into four main groups: (i) PRGF membrane (M-PRGF), (ii) PRGF supernatant (S-PRGF), (iii) platelet-poor plasma (PPP) membrane diluted 50% with S-PRGF (M-PPP 50%), and (iv) M-PPP 50% supernatant (S-PPP 50%). ARPE-19 cells were exposed to blue light and then incubated with the different PRGF-derived formulations or control for 24 and 48 h under blue light exposure. Mitochondrial and cell viability, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) and ZO-1 expression were evaluated. Mitochondrial viability and cell survival were significantly increased after treatment with the different PRGF-derived formulations. ROS synthesis and HO-1 expression were significantly reduced after cell treatment with any of the PRGF-derived formulations. Furthermore, the different PRGF-derived formulations significantly increased ZO-1 expression in ARPE-19 exposed to blue light. The new PRGF membrane with improved optical properties and its supernatant (M-PPP 50% and S-PPP 50%) protected and reversed blue light-induced oxidative stress in ARPE-19 cells at levels like those of a natural PRGF membrane and its supernatant.
Keywords: platelet rich plasma; PRGF; oxidative stress; age-related macular degeneration; AMD; retinal pigment epithelial cells; transparence; growth factors platelet rich plasma; PRGF; oxidative stress; age-related macular degeneration; AMD; retinal pigment epithelial cells; transparence; growth factors

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MDPI and ACS Style

Anitua, E.; Muruzabal, F.; de la Fuente, M.; Del Olmo-Aguado, S.; Alkhraisat, M.H.; Merayo-Lloves, J. PRGF Membrane with Tailored Optical Properties Preserves the Cytoprotective Effect of Plasma Rich in Growth Factors: In Vitro Model of Retinal Pigment Epithelial Cells. Int. J. Mol. Sci. 2023, 24, 11195. https://doi.org/10.3390/ijms241311195

AMA Style

Anitua E, Muruzabal F, de la Fuente M, Del Olmo-Aguado S, Alkhraisat MH, Merayo-Lloves J. PRGF Membrane with Tailored Optical Properties Preserves the Cytoprotective Effect of Plasma Rich in Growth Factors: In Vitro Model of Retinal Pigment Epithelial Cells. International Journal of Molecular Sciences. 2023; 24(13):11195. https://doi.org/10.3390/ijms241311195

Chicago/Turabian Style

Anitua, Eduardo, Francisco Muruzabal, María de la Fuente, Susana Del Olmo-Aguado, Mohammad H. Alkhraisat, and Jesús Merayo-Lloves. 2023. "PRGF Membrane with Tailored Optical Properties Preserves the Cytoprotective Effect of Plasma Rich in Growth Factors: In Vitro Model of Retinal Pigment Epithelial Cells" International Journal of Molecular Sciences 24, no. 13: 11195. https://doi.org/10.3390/ijms241311195

APA Style

Anitua, E., Muruzabal, F., de la Fuente, M., Del Olmo-Aguado, S., Alkhraisat, M. H., & Merayo-Lloves, J. (2023). PRGF Membrane with Tailored Optical Properties Preserves the Cytoprotective Effect of Plasma Rich in Growth Factors: In Vitro Model of Retinal Pigment Epithelial Cells. International Journal of Molecular Sciences, 24(13), 11195. https://doi.org/10.3390/ijms241311195

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