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Article

Comparison of Enzyme Activity in Order to Describe the Metabolic Profile of Dairy Cows during Early Lactation

by
Kamila Puppel
*,
Jan Slósarz
,
Grzegorz Grodkowski
,
Paweł Solarczyk
,
Piotr Kostusiak
,
Małgorzata Kunowska-Slósarz
,
Kinga Grodkowska
,
Anna Zalewska
,
Beata Kuczyńska
and
Marcin Gołębiewski
Institute of Animal Sciences, Departments of Animal Breeding, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(17), 9771; https://doi.org/10.3390/ijms23179771
Submission received: 25 July 2022 / Revised: 24 August 2022 / Accepted: 25 August 2022 / Published: 29 August 2022
(This article belongs to the Section Molecular Endocrinology and Metabolism)
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Abstract

Enzymatic diagnostics have practical applications in diseases of the liver, heart, pancreas, muscles, blood, and neoplastic diseases. This study aimed to compare enzyme activity to describe dairy cows’ metabolism during early lactation. Based on their general health symptoms, the cows were assigned to one of three groups: acidotic, healthy and ketotic. Samples of milk, blood and rumen fluid were collected at 12 ± 5 days postpartum. Ketotic cows were characterized by the highest malondialdehyde (MDA, 76.098 nM/mL), glutathione reductase (GluRed, 109.852 U/L), superoxide dismutase (SOD, 294.22 U/L) and gamma-glutamyltranspeptidase (GGTP, 71.175 U/L) activity. In comparing ketotic and acidotic cows, MDA, GluRed, SOD and GGTP activity were higher by a factor of almost: 1.85, 1.89, 0.79 and 2.50, respectively. Acidotic cows were characterized by the highest aspartate aminotransferase activity (AspAT, 125.914 U/L). In comparing acidotic and ketotic cows, AspAT activity was higher by a factor of almost 1.90. The use of enzymatic markers could limit the frequency of sampling for laboratory analyses and may result in a faster diagnosis of metabolic disorders. AspAT activity in blood serum seems to be a good indicator of acidosis; GGTP may participate in the pathogenesis of ketosis.
Keywords: enzymes; ketosis; acidosis; cow; AspAT; GGTP enzymes; ketosis; acidosis; cow; AspAT; GGTP

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MDPI and ACS Style

Puppel, K.; Slósarz, J.; Grodkowski, G.; Solarczyk, P.; Kostusiak, P.; Kunowska-Slósarz, M.; Grodkowska, K.; Zalewska, A.; Kuczyńska, B.; Gołębiewski, M. Comparison of Enzyme Activity in Order to Describe the Metabolic Profile of Dairy Cows during Early Lactation. Int. J. Mol. Sci. 2022, 23, 9771. https://doi.org/10.3390/ijms23179771

AMA Style

Puppel K, Slósarz J, Grodkowski G, Solarczyk P, Kostusiak P, Kunowska-Slósarz M, Grodkowska K, Zalewska A, Kuczyńska B, Gołębiewski M. Comparison of Enzyme Activity in Order to Describe the Metabolic Profile of Dairy Cows during Early Lactation. International Journal of Molecular Sciences. 2022; 23(17):9771. https://doi.org/10.3390/ijms23179771

Chicago/Turabian Style

Puppel, Kamila, Jan Slósarz, Grzegorz Grodkowski, Paweł Solarczyk, Piotr Kostusiak, Małgorzata Kunowska-Slósarz, Kinga Grodkowska, Anna Zalewska, Beata Kuczyńska, and Marcin Gołębiewski. 2022. "Comparison of Enzyme Activity in Order to Describe the Metabolic Profile of Dairy Cows during Early Lactation" International Journal of Molecular Sciences 23, no. 17: 9771. https://doi.org/10.3390/ijms23179771

APA Style

Puppel, K., Slósarz, J., Grodkowski, G., Solarczyk, P., Kostusiak, P., Kunowska-Slósarz, M., Grodkowska, K., Zalewska, A., Kuczyńska, B., & Gołębiewski, M. (2022). Comparison of Enzyme Activity in Order to Describe the Metabolic Profile of Dairy Cows during Early Lactation. International Journal of Molecular Sciences, 23(17), 9771. https://doi.org/10.3390/ijms23179771

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