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Open AccessArticle

Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application

1
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, D-07743 Jena, Germany
2
Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Albert-Einstein-Straße 9, D-07745 Jena, Germany
3
Department of Cardiology Internal Medicine, Jena University Hospital, Am Klinikum 1, D-07747 Jena, Germany
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Mid-German Heart Center, Department of Internal Medicine III (KIM-III), Division of Cardiology, Angiology and Intensive Medical Care, University Hospital Halle, Martin-Luther-University Halle-Wittenberg, Ernst-Grube-Strasse 40, D-06120 Halle (Saale), Germany
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Center for Sepsis Control and Care (CSCC), Jena University Hospital, Am Klinikum 1, D-07747 Jena, Germany
6
InfectoGnostics Research Campus Jena, Centre of Applied Research, Philosophenweg 7, D-07743 Jena, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Chan-Gi Pack
Int. J. Mol. Sci. 2021, 22(4), 2191; https://doi.org/10.3390/ijms22042191
Received: 21 January 2021 / Revised: 13 February 2021 / Accepted: 19 February 2021 / Published: 22 February 2021
In recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vibrational signal. However, most often encountered challenge is the inhomogeneous redistribution of biomolecules due to the coffee-ring effect. In this study, the variation in biomolecule distribution within the dried-sample droplet has been investigated using Raman and FT-IR spectroscopy and fluorescence lifetime imaging method. The plasma-sample from healthy donors were investigated to show the spectral differences between the inner and outer-ring region of the dried-sample droplet. Further, the preferred location of deposition of the most abundant protein albumin in the blood during the drying process of the plasma has been illustrated by using deuterated albumin. Subsequently, two patients with different cardiac-related diseases were investigated exemplarily to illustrate the variation in the pattern of plasma and serum biomolecule distribution during the drying process and its impact on patient-stratification. The study shows that a uniform sampling position of the droplet, both at the inner and the outer ring, is necessary for thorough clinical characterization of the patient’s plasma and serum sample using vibrational spectroscopy. View Full-Text
Keywords: plasma; serum; coffee-ring effect; cardiac patients; vibrational spectroscopy; fluorescence lifetime plasma; serum; coffee-ring effect; cardiac patients; vibrational spectroscopy; fluorescence lifetime
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MDPI and ACS Style

Huang, J.; Ali, N.; Quansah, E.; Guo, S.; Noutsias, M.; Meyer-Zedler, T.; Bocklitz, T.; Popp, J.; Neugebauer, U.; Ramoji, A. Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application. Int. J. Mol. Sci. 2021, 22, 2191. https://doi.org/10.3390/ijms22042191

AMA Style

Huang J, Ali N, Quansah E, Guo S, Noutsias M, Meyer-Zedler T, Bocklitz T, Popp J, Neugebauer U, Ramoji A. Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application. International Journal of Molecular Sciences. 2021; 22(4):2191. https://doi.org/10.3390/ijms22042191

Chicago/Turabian Style

Huang, Jing; Ali, Nairveen; Quansah, Elsie; Guo, Shuxia; Noutsias, Michel; Meyer-Zedler, Tobias; Bocklitz, Thomas; Popp, Jürgen; Neugebauer, Ute; Ramoji, Anuradha. 2021. "Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application" Int. J. Mol. Sci. 22, no. 4: 2191. https://doi.org/10.3390/ijms22042191

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