Next Article in Journal
Integrin Regulation in Immunological and Cancerous Cells and Exosomes
Next Article in Special Issue
OsBRKq1, Related Grain Size Mapping, and Identification of Grain Shape Based on QTL Mapping in Rice
Previous Article in Journal
Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application
Previous Article in Special Issue
OsCRP1, a Ribonucleoprotein Gene, Regulates Chloroplast mRNA Stability That Confers Drought and Cold Tolerance
Open AccessArticle

New Insights into the Transcriptional Regulation of Genes Involved in the Nitrogen Use Efficiency under Potassium Chlorate in Rice (Oryza sativa L.)

1
Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea
2
Molecular Biology Department, Agricultural Genetic Institute, Hanoi 11917, Vietnam
*
Author to whom correspondence should be addressed.
Academic Editor: Kiyosumi Hori
Int. J. Mol. Sci. 2021, 22(4), 2192; https://doi.org/10.3390/ijms22042192
Received: 28 January 2021 / Revised: 16 February 2021 / Accepted: 17 February 2021 / Published: 22 February 2021
(This article belongs to the Special Issue Molecular Research in Rice: Agronomically Important Traits 2.0)
Potassium chlorate (KClO3) has been widely used to evaluate the divergence in nitrogen use efficiency (NUE) between indica and japonica rice subspecies. This study investigated the transcriptional regulation of major genes involved in the NUE in rice treated with KClO3, which acts as an inhibitor of the reducing activity of nitrate reductase (NR) in higher plants. A set of two KClO3 sensitive nitrate reductase (NR) and two nitrate transporter (NRT) introgression rice lines (BC2F7), carrying the indica alleles of NR or NRT, derived from a cross between Saeilmi (japonica, P1) and Milyang23 (indica, P2), were exposed to KClO3 at the seedling stage. The phenotypic responses were recorded 7 days after treatment, and samples for gene expression, physiological, and biochemical analyses were collected at 0 h (control) and 3 h after KClO3 application. The results revealed that Saeilmi (P1, japonica) and Milyang23 (P2, indica) showed distinctive phenotypic responses. In addition, the expression of OsNR2 was differentially regulated between the roots, stem, and leaf tissues, and between introgression lines. When expressed in the roots, OsNR2 was downregulated in all introgression lines. However, in the stem and leaves, OsNR2 was upregulated in the NR introgression lines, but downregulation in the NRT introgression lines. In the same way, the expression patterns of OsNIA1 and OsNIA2 in the roots, stem, and leaves indicated a differential transcriptional regulation by KClO3, with OsNIA2 prevailing over OsNIA1 in the roots. Under the same conditions, the activity of NR was inhibited in the roots and differentially regulated in the stem and leaf tissues. Furthermore, the transcriptional divergence of OsAMT1.3 and OsAMT2.3, OsGLU1 and OsGLU2, between NR and NRT, coupled with the NR activity pattern in the roots, would indicate the prevalence of nitrate (NO3¯) transport over ammonium (NH4+) transport. Moreover, the induction of catalase (CAT) and polyphenol oxidase (PPO) enzyme activities in Saeilmi (P1, KClO3 resistant), and the decrease in Milyang23 (P2, KClO3 sensitive), coupled with the malondialdehyde (MDA) content, indicated the extent of the oxidative stress, and the induction of the adaptive response mechanism, tending to maintain a balanced reduction–oxidation state in response to KClO3. The changes in the chloroplast pigments and proline content propose these compounds as emerging biomarkers for assessing the overall plant health status. These results suggest that the inhibitory potential of KClO3 on the reduction activity of the nitrate reductase (NR), as well as that of the genes encoding the nitrate and ammonium transporters, and glutamate synthase are tissue-specific, which may differentially affect the transport and assimilation of nitrate or ammonium in rice. View Full-Text
Keywords: nitrogen use efficiency; transcriptional regulation; nitrate reductase; nitrate transporter; glutamate synthase; potassium chlorate; rice nitrogen use efficiency; transcriptional regulation; nitrate reductase; nitrate transporter; glutamate synthase; potassium chlorate; rice
Show Figures

Figure 1

MDPI and ACS Style

Kabange, N.R.; Park, S.-Y.; Lee, J.-Y.; Shin, D.; Lee, S.-M.; Kwon, Y.; Cha, J.-K.; Cho, J.-H.; Duyen, D.V.; Ko, J.-M.; Lee, J.-H. New Insights into the Transcriptional Regulation of Genes Involved in the Nitrogen Use Efficiency under Potassium Chlorate in Rice (Oryza sativa L.). Int. J. Mol. Sci. 2021, 22, 2192. https://doi.org/10.3390/ijms22042192

AMA Style

Kabange NR, Park S-Y, Lee J-Y, Shin D, Lee S-M, Kwon Y, Cha J-K, Cho J-H, Duyen DV, Ko J-M, Lee J-H. New Insights into the Transcriptional Regulation of Genes Involved in the Nitrogen Use Efficiency under Potassium Chlorate in Rice (Oryza sativa L.). International Journal of Molecular Sciences. 2021; 22(4):2192. https://doi.org/10.3390/ijms22042192

Chicago/Turabian Style

Kabange, Nkulu R.; Park, So-Yeon; Lee, Ji-Yun; Shin, Dongjin; Lee, So-Myeong; Kwon, Youngho; Cha, Jin-Kyung; Cho, Jun-Hyeon; Duyen, Dang V.; Ko, Jong-Min; Lee, Jong-Hee. 2021. "New Insights into the Transcriptional Regulation of Genes Involved in the Nitrogen Use Efficiency under Potassium Chlorate in Rice (Oryza sativa L.)" Int. J. Mol. Sci. 22, no. 4: 2192. https://doi.org/10.3390/ijms22042192

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop