Interleukin-17 Reduces βENaC via MAPK Signaling in Vascular Smooth Muscle Cells
Abstract
:1. Introduction
2. Methods
2.1. Cell Culture
2.2. Cell Treatment
2.3. Protein Isolation
2.4. Western Blot on A10 Protein Isolates
2.5. Live/Dead Viability/Cytotoxicity Assay
2.6. Statistical Analysis.
3. Results
3.1. IL-17 Reduces βENaC Expression in a Concentration-Dependent Fashion in Cultured VSMCs
3.2. Reduction in βENaC by IL-17 Is Not Associated with Cell Death
3.3. IL-17 Induces the Phosphorylation of p38MAPK and JNK, but Not NFκB
3.4. Role of p38MAPK and JNK in the Reduction of βENaC by IL-17
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Duncan, J.W.; Granger, J.P.; Ryan, M.J.; Drummond, H.A. Interleukin-17 Reduces βENaC via MAPK Signaling in Vascular Smooth Muscle Cells. Int. J. Mol. Sci. 2020, 21, 2953. https://doi.org/10.3390/ijms21082953
Duncan JW, Granger JP, Ryan MJ, Drummond HA. Interleukin-17 Reduces βENaC via MAPK Signaling in Vascular Smooth Muscle Cells. International Journal of Molecular Sciences. 2020; 21(8):2953. https://doi.org/10.3390/ijms21082953
Chicago/Turabian StyleDuncan, Jeremy W., Joey P. Granger, Michael J. Ryan, and Heather A. Drummond. 2020. "Interleukin-17 Reduces βENaC via MAPK Signaling in Vascular Smooth Muscle Cells" International Journal of Molecular Sciences 21, no. 8: 2953. https://doi.org/10.3390/ijms21082953