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Article

Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins

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Nano-Biotechnology Group, Department of Biotechology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium
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Engineering Department., Lancaster University, Gillow Avenue, Lancaster LA1 4YW, UK
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Materials Science Institute (MSI), Lancaster University, Lancaster LA1 4YW, UK
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Division of Food Technology, Kiel University, 24118 Kiel, Germany
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Laboratory of Food Process Engineering, Wageningen University & Research AFSG, 6708 PB Wageningen, The Netherlands
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Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, 142 00 Prague, Czech Republic
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Department of Genetics and Microbiology, Charles University in Prague, 116 36 Prague, Czech Republic
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Department of Nutritional and Food Sciences, University of Bonn, D-53012 Bonn, Germany
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FSRC “Crystallography and photonics” RAS, 119333 Moscow, Russia
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Institute of Nanostructures and Biosystems, Saratov NG Chernyshevskii State Univ, 83 Astrakhanskaya St, 410012 Saratov, Russia
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Omnichem NV, 9230 Wetterem, Belgium
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Department of Chemistry, Ghent University, Krijgslaan 281, 9000 Ghent, Belgium
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Centre for Nano- and Biophotonics, Ghent University, 9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(7), 2315; https://doi.org/10.3390/ijms21072315
Received: 30 December 2019 / Revised: 17 March 2020 / Accepted: 21 March 2020 / Published: 27 March 2020
(This article belongs to the Special Issue Advanced Polymer Composite Materials)
Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols. Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.). Certain gallotannin preparations promoted mineralization to a greater degree than others. The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization. Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels. Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity. In conclusion, gallotannin incorporation aided mineralization but reduced cytocompatibility. View Full-Text
Keywords: mineralization; polyphenol; composite; protein-polyphenol interaction; gellan gum; enzyme mineralization; polyphenol; composite; protein-polyphenol interaction; gellan gum; enzyme
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MDPI and ACS Style

Douglas, T.E.L.; Keppler, J.K.; Vandrovcová, M.; Plencner, M.; Beranová, J.; Feuereisen, M.; Parakhonskiy, B.V.; Svenskaya, Y.; Atkin, V.; Ivanova, A.; Ricquier, P.; Balcaen, L.; Vanhaecke, F.; Schieber, A.; Bačáková, L.; Skirtach, A.G. Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins. Int. J. Mol. Sci. 2020, 21, 2315. https://doi.org/10.3390/ijms21072315

AMA Style

Douglas TEL, Keppler JK, Vandrovcová M, Plencner M, Beranová J, Feuereisen M, Parakhonskiy BV, Svenskaya Y, Atkin V, Ivanova A, Ricquier P, Balcaen L, Vanhaecke F, Schieber A, Bačáková L, Skirtach AG. Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins. International Journal of Molecular Sciences. 2020; 21(7):2315. https://doi.org/10.3390/ijms21072315

Chicago/Turabian Style

Douglas, Timothy E. L., Julia K. Keppler, Marta Vandrovcová, Martin Plencner, Jana Beranová, Michelle Feuereisen, Bogdan V. Parakhonskiy, Yulia Svenskaya, Vsevolod Atkin, Anna Ivanova, Patrick Ricquier, Lieve Balcaen, Frank Vanhaecke, Andreas Schieber, Lucie Bačáková, and Andre G. Skirtach. 2020. "Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins" International Journal of Molecular Sciences 21, no. 7: 2315. https://doi.org/10.3390/ijms21072315

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