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Article

LreEF1A4, a Translation Elongation Factor from Lilium regale, Is Pivotal for Cucumber Mosaic Virus and Tobacco Rattle Virus Infections and Tolerance to Salt and Drought

1
College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, China
2
State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth 6150, Australia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(6), 2083; https://doi.org/10.3390/ijms21062083
Received: 20 February 2020 / Revised: 14 March 2020 / Accepted: 16 March 2020 / Published: 18 March 2020
(This article belongs to the Special Issue Plant Disease Resistance)
Eukaryotic translation elongation factors are implicated in protein synthesis across different living organisms, but their biological functions in the pathogenesis of cucumber mosaic virus (CMV) and tobacco rattle virus (TRV) infections are poorly understood. Here, we isolated and characterized a cDNA clone, LreEF1A4, encoding the alpha subunit of elongation factor 1, from a CMV-elicited suppression subtractive hybridization library of Lilium regale. The infection tests using CMV remarkably increased transcript abundance of LreEF1A4; however, it also led to inconsistent expression profiles of three other LreEF1A homologs (LreEF1A1–3). Protein modelling analysis revealed that the amino acid substitutions among four LreEF1As may not affect their enzymatic functions. LreEF1A4 was ectopically overexpressed in petunia (Petunia hybrida), and transgenic plants exhibited delayed leaf and flower senescence, concomitant with increased transcription of photosynthesis-related genes and reduced expression of senescence-associated genes, respectively. A compromised resistance to CMV and TRV infections was found in transgenic petunia plants overexpressing LreEF1A4, whereas its overexpression resulted in an enhanced tolerance to salt and drought stresses. Taken together, our data demonstrate that LreEF1A4 functions as a positive regulator in viral multiplication and plant adaption to high salinity and dehydration. View Full-Text
Keywords: eukaryotic translation elongation factor; Lilium regale; petunia; cucumber mosaic virus; tobacco rattle virus; salt; drought eukaryotic translation elongation factor; Lilium regale; petunia; cucumber mosaic virus; tobacco rattle virus; salt; drought
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MDPI and ACS Style

Sun, D.; Ji, X.; Jia, Y.; Huo, D.; Si, S.; Zeng, L.; Zhang, Y.; Niu, L. LreEF1A4, a Translation Elongation Factor from Lilium regale, Is Pivotal for Cucumber Mosaic Virus and Tobacco Rattle Virus Infections and Tolerance to Salt and Drought. Int. J. Mol. Sci. 2020, 21, 2083. https://doi.org/10.3390/ijms21062083

AMA Style

Sun D, Ji X, Jia Y, Huo D, Si S, Zeng L, Zhang Y, Niu L. LreEF1A4, a Translation Elongation Factor from Lilium regale, Is Pivotal for Cucumber Mosaic Virus and Tobacco Rattle Virus Infections and Tolerance to Salt and Drought. International Journal of Molecular Sciences. 2020; 21(6):2083. https://doi.org/10.3390/ijms21062083

Chicago/Turabian Style

Sun, Daoyang; Ji, Xiaotong; Jia, Yong; Huo, Dan; Si, Shiying; Zeng, Lingling; Zhang, Yanlong; Niu, Lixin. 2020. "LreEF1A4, a Translation Elongation Factor from Lilium regale, Is Pivotal for Cucumber Mosaic Virus and Tobacco Rattle Virus Infections and Tolerance to Salt and Drought" Int. J. Mol. Sci. 21, no. 6: 2083. https://doi.org/10.3390/ijms21062083

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