Loss of ISWI ATPase SMARCA5 (SNF2H) in Acute Myeloid Leukemia Cells Inhibits Proliferation and Chromatid Cohesion
Abstract
:1. Introduction
2. Results
2.1. SMARCA5 Overexpression Marks the Hyperproliferation and Cytogenetically Abnormal AML Patients
2.2. SMARCA5 Deletion Inhibits AML Cell Proliferation
2.3. Smarca5 Deletion Inhibits Proliferation of Myeloblasts and Affects Function of Normal Stem Cells
2.4. Inactivation of Smarca5 Causes Nuclear Abnormalities and Polyploidy
2.5. Cytogenetic Abnormalities and Gene Expression Dysregulation in the S5KO AML Cells
3. Discussion
4. Materials and Methods
4.1. CRISPR Vector Design
4.2. Cell Lines
4.3. AML Patients and Statistics
4.4. Real-Time qPCR
4.5. Western Blot
4.6. Cytogenetics
4.7. Hematopoietic Reconstitution
4.8. Analysis of S5KO MEF Cells
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
SMARCA5 | SWI/SNF-Related, Matrix-Associated, Actin-Dependent Regulator of Chromatin, Subfamily A, Member 5 |
SNF2H | Sucrose Nonfermenting Protein 2 Homolog |
AML | Acute Myeloid Leukemia |
CRISPR | Clustered Regularly Interspaced Short Palindromic Repeats |
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Zikmund, T.; Paszekova, H.; Kokavec, J.; Kerbs, P.; Thakur, S.; Turkova, T.; Tauchmanova, P.; Greif, P.A.; Stopka, T. Loss of ISWI ATPase SMARCA5 (SNF2H) in Acute Myeloid Leukemia Cells Inhibits Proliferation and Chromatid Cohesion. Int. J. Mol. Sci. 2020, 21, 2073. https://doi.org/10.3390/ijms21062073
Zikmund T, Paszekova H, Kokavec J, Kerbs P, Thakur S, Turkova T, Tauchmanova P, Greif PA, Stopka T. Loss of ISWI ATPase SMARCA5 (SNF2H) in Acute Myeloid Leukemia Cells Inhibits Proliferation and Chromatid Cohesion. International Journal of Molecular Sciences. 2020; 21(6):2073. https://doi.org/10.3390/ijms21062073
Chicago/Turabian StyleZikmund, Tomas, Helena Paszekova, Juraj Kokavec, Paul Kerbs, Shefali Thakur, Tereza Turkova, Petra Tauchmanova, Philipp A. Greif, and Tomas Stopka. 2020. "Loss of ISWI ATPase SMARCA5 (SNF2H) in Acute Myeloid Leukemia Cells Inhibits Proliferation and Chromatid Cohesion" International Journal of Molecular Sciences 21, no. 6: 2073. https://doi.org/10.3390/ijms21062073