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Open AccessArticle

Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis

1
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo náměstí 2, 166 10 Prague, Czech Republic
2
Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 2030, 128 43 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Current address: University College London, Gower St, London WC1E 6B, UK.
Int. J. Mol. Sci. 2020, 21(3), 850; https://doi.org/10.3390/ijms21030850
Received: 17 December 2019 / Revised: 21 January 2020 / Accepted: 24 January 2020 / Published: 28 January 2020
(This article belongs to the Special Issue Carbonic Anhydrase and Biomarker Research)
Pathogenic yeasts Candida albicans and Candida parapsilosis possess a ß-type carbonic anhydrase Nce103p, which is involved in CO2 hydration and signaling. C. albicans lacking Nce103p cannot survive in low CO2 concentrations, e.g., in atmospheric growth conditions. Candida carbonic anhydrases are orthologous to the Saccharomyces cerevisiae enzyme, which had originally been detected as a substrate of a non-classical export pathway. However, experimental evidence on localization of C. albicans and C. parapsilosis carbonic anhydrases has not been reported to date. Immunogold labeling and electron microscopy used in the present study showed that carbonic anhydrases are localized in the cell wall and plasmatic membrane of both Candida species. This localization was confirmed by Western blot and mass spectrometry analyses of isolated cell wall and plasma membrane fractions. Further analysis of C. albicans and C. parapsilosis subcellular fractions revealed presence of carbonic anhydrases also in the cytosolic and mitochondrial fractions of Candida cells cultivated in shaken liquid cultures, under the atmospheric conditions. View Full-Text
Keywords: carbonic anhydrase; Nce103p; Candida albicans; Candida parapsilosis; localization; cell wall; electron microscopy; mass spectrometry carbonic anhydrase; Nce103p; Candida albicans; Candida parapsilosis; localization; cell wall; electron microscopy; mass spectrometry
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MDPI and ACS Style

Dostál, J.; Blaha, J.; Hadravová, R.; Hubálek, M.; Heidingsfeld, O.; Pichová, I. Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis. Int. J. Mol. Sci. 2020, 21, 850.

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