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Article

A Pentapeptide with Tyrosine Moiety as Fluorescent Chemosensor for Selective Nanomolar-Level Detection of Copper(II) Ions

Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland
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Int. J. Mol. Sci. 2020, 21(3), 743; https://doi.org/10.3390/ijms21030743
Received: 27 November 2019 / Revised: 17 January 2020 / Accepted: 21 January 2020 / Published: 23 January 2020
(This article belongs to the Section Molecular Biophysics)
Herein, we have investigated principally with the use of UV and fluorescence (steady-state and time-resolved) spectroscopy the interactions between selected pentapeptides with tyrosine residue (EYHHQ, EHYHQ, EHHQY, and KYHHE) and various metal ions (Cu2+, Mn2+, Co2+, Ni2+, Zn2+, Cr3+, Cd2+, Ag+, Pb2+, Sr2+, Ba2+, Ca2+, Mg2+, Al3+, Fe2+, and Ga3+) in order to establish the relationship between the position of a tyrosine residue in the peptide sequence and the metal ion-binding properties. Among the peptides studied, EHYHQ was evaluated as an efficient and selective ligand for developing a chemosensor for the detection of copper(II) ions. While significant fluorescence emission quenching was observed for that peptide in the presence of Cu2+ cations, other metal cations used at the same and at considerably higher concentrations caused a negligible change of the fluorescence emission spectrum, indicating a high selectivity of EHYHQ for Cu2+ ions. Under optimum conditions, fluorescence intensity was inversely proportional to the concentration of Cu2+ ions. The limit of detection of Cu2+ ions with the use of EHYHQ was determined at the level of 26.6 nM. The binding stoichiometry of the complexes of the studied peptides with Cu2+ ions was evaluated spectrophotometrically and fluorimetrically (as in the case of EHYHQ confirmed by mass spectrometry) and found to be 1:2 (Cu2+-peptide) for all the investigated systems. Furthermore, the stability constant (K) values of these complexes were determined. The reversibility of the proposed Cu2+ ions sensor was confirmed, the pH range where the sensor acts was determined, while its analytical performance was compared with some other reported recently fluorescent sensors. The mechanism of the interactions between EHYHQ and Cu2+ was proposed on the basis of NMR spectroscopy investigations. View Full-Text
Keywords: Cu2+ ions; fluorescent chemosensor; pentapeptides; fluorescence quenching Cu2+ ions; fluorescent chemosensor; pentapeptides; fluorescence quenching
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MDPI and ACS Style

Żamojć, K.; Kamrowski, D.; Zdrowowicz, M.; Wyrzykowski, D.; Wiczk, W.; Chmurzyński, L.; Makowska, J. A Pentapeptide with Tyrosine Moiety as Fluorescent Chemosensor for Selective Nanomolar-Level Detection of Copper(II) Ions. Int. J. Mol. Sci. 2020, 21, 743. https://doi.org/10.3390/ijms21030743

AMA Style

Żamojć K, Kamrowski D, Zdrowowicz M, Wyrzykowski D, Wiczk W, Chmurzyński L, Makowska J. A Pentapeptide with Tyrosine Moiety as Fluorescent Chemosensor for Selective Nanomolar-Level Detection of Copper(II) Ions. International Journal of Molecular Sciences. 2020; 21(3):743. https://doi.org/10.3390/ijms21030743

Chicago/Turabian Style

Żamojć, Krzysztof, Dominik Kamrowski, Magdalena Zdrowowicz, Dariusz Wyrzykowski, Wiesław Wiczk, Lech Chmurzyński, and Joanna Makowska. 2020. "A Pentapeptide with Tyrosine Moiety as Fluorescent Chemosensor for Selective Nanomolar-Level Detection of Copper(II) Ions" International Journal of Molecular Sciences 21, no. 3: 743. https://doi.org/10.3390/ijms21030743

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