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Deep Sequencing MicroRNAs from Extracellular Membrane Vesicles Revealed the Association of the Vesicle Cargo with Cellular Origin

1
Vinmec Research Institute of Stem Cell and Gene Technology, Vinmec Health Care System, Ha Noi 10000, Vietnam
2
Tissue Repair and Translational Physiology Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia
3
School of Biomedical Science, Faculty of Health, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia
4
Skin Research Institute of Singapore, Agency for Science, Technology and Research, 8A Biomedical Grove, Singapore 138648, Singapore
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 1141; https://doi.org/10.3390/ijms21031141
Received: 20 January 2020 / Revised: 1 February 2020 / Accepted: 4 February 2020 / Published: 8 February 2020
(This article belongs to the Section Molecular Biology)
Extracellular membrane vesicles (EVs) have emerged as potential candidates for diagnostics and therapeutics. We have previously reported that keratinocytes release three types of EVs into the extracellular environment. Importantly, those EVs contain a large number of microRNAs (miRNAs) as cargo. In this study, we examined the expression level of keratinocyte-derived EV miRNAs, their target genes and potential functions. Next generation sequencing results showed that over one hundred miRNAs in each EV subtype exhibited greater than 100 reads per million (RPM), indicating a relatively high abundance. Analysis of the miRNAs with the highest abundance revealed associations with different keratinocyte cell sources. For instance, hsa-miR-205 was associated with the HaCaT cells whereas hsa-miR-21, hsa-miR-203, hsa-miR-22 and hsa-miR-143 were associated with human primary dermal keratinocytes (PKCs). Additionally, functional annotation analysis of genes regulated by those miRNAs, especially with regard to biological processes, also revealed cell-type-specific associations with either HaCaTs or PKCs. Indeed, EV functional effects were related to their parental cellular origin; specifically, PKC-derived EVs influenced fibroblast migration whereas HaCaT-derived EVs did not. In addition, the data in this current study indicates that keratinocyte-derived EVs and/or their cargoes have potential applications for wound healing. View Full-Text
Keywords: microRNA; extracellular membrane vesicle; keratinocytes; wound healing microRNA; extracellular membrane vesicle; keratinocytes; wound healing
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Than, U.T.T.; Guanzon, D.; Broadbent, J.A.; Parker, T.J.; Leavesley, D.I. Deep Sequencing MicroRNAs from Extracellular Membrane Vesicles Revealed the Association of the Vesicle Cargo with Cellular Origin. Int. J. Mol. Sci. 2020, 21, 1141.

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