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In Vitro Tissue Culture in Brachypodium: Applications and Challenges

1
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 28 Jagiellonska Street, 40-032 Katowice, Poland
2
National Plant Phenomics Centre, IBERS, Aberystwyth University, Aberystwyth SY23 3EE, UK
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 1037; https://doi.org/10.3390/ijms21031037
Received: 18 December 2019 / Revised: 1 February 2020 / Accepted: 3 February 2020 / Published: 4 February 2020
(This article belongs to the Special Issue Plant Cell and Organism Development)
Brachypodium distachyon has become an excellent model for plant breeding and bioenergy grasses that permits many fundamental questions in grass biology to be addressed. One of the constraints to performing research in many grasses has been the difficulty with which they can be genetically transformed and the generally low frequency of such transformations. In this review, we discuss the contribution that transformation techniques have made in Brachypodium biology as well as how Brachypodium could be used to determine the factors that might contribute to transformation efficiency. In particular, we highlight the latest research on the mechanisms that govern the gradual loss of embryogenic potential in a tissue culture and propose using B. distachyon as a model for other recalcitrant monocots. View Full-Text
Keywords: Agrobacterium; Brachypodium distachyon; Brachypodium species; cell wall; genes; model plant; somatic embryogenesis; transformation Agrobacterium; Brachypodium distachyon; Brachypodium species; cell wall; genes; model plant; somatic embryogenesis; transformation
MDPI and ACS Style

Betekhtin, A.; Hus, K.; Rojek-Jelonek, M.; Kurczynska, E.; Nibau, C.; Doonan, J.H.; Hasterok, R. In Vitro Tissue Culture in Brachypodium: Applications and Challenges. Int. J. Mol. Sci. 2020, 21, 1037.

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