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Article

A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues

1
KOSÉ R&D France, KOSÉ Corporation, 5 Avenue Lionel Terray, 69330 Meyzieu, France
2
Research Laboratories, KOSÉ Corporation, 48-18 Sakae-cho, Kita-ku, Tokyo 114-0005, Japan
3
Laboratory of Membrane Trafficking Mechanisms, Department of Integrative Life Sciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8514; https://doi.org/10.3390/ijms21228514
Received: 23 October 2020 / Revised: 6 November 2020 / Accepted: 9 November 2020 / Published: 12 November 2020
(This article belongs to the Special Issue Melanosome Transport/Transfer and Melanin Pigmentation in the Skin)
Melanin incorporated into keratinocytes plays an important role in photoprotection; however, abnormal melanin accumulation causes hyperpigmentary disorders. To understand the mechanism behind the accumulation of excess melanin in the skin, it is essential to clarify the spatial distribution of melanosomes or melanin in the epidermis. Although several markers have been used to detect melanosomes or melanin, no suitable markers to determine the precise localization of melanin in the epidermis have been reported. In this study, we showed that melanocore-interacting Kif1c-tail (M-INK), a recently developed fluorescent probe for visualizing mature melanosomes, binds to purified melanin in vitro, and applied it for detecting melanin in human skin tissues. Frozen skin sections from different phototypes were co-stained for the hemagglutinin (HA)-tagged M-INK probe and markers of melanocytes or keratinocytes, and a wide distribution of melanin was observed in the epidermis. Analysis of the different skin phototypes indicated that the fluorescent signals of HA-M-INK correlated well with skin color. The reconstruction of three-dimensional images of epidermal sheets enabled us to observe the spatial distribution of melanin in the epidermis. Thus, the HA-M-INK probe is an ideal tool to individually visualize melanin (or melanosome) distribution in melanocytes and in keratinocytes in skin tissues. View Full-Text
Keywords: human skin; keratinocyte; melanin distribution; melanocyte; melanosome; M-INK; skin phototype; three-dimensional observation human skin; keratinocyte; melanin distribution; melanocyte; melanosome; M-INK; skin phototype; three-dimensional observation
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MDPI and ACS Style

Yoshikawa-Murakami, C.; Mizutani, Y.; Ryu, A.; Naru, E.; Teramura, T.; Homma, Y.; Fukuda, M. A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues. Int. J. Mol. Sci. 2020, 21, 8514. https://doi.org/10.3390/ijms21228514

AMA Style

Yoshikawa-Murakami C, Mizutani Y, Ryu A, Naru E, Teramura T, Homma Y, Fukuda M. A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues. International Journal of Molecular Sciences. 2020; 21(22):8514. https://doi.org/10.3390/ijms21228514

Chicago/Turabian Style

Yoshikawa-Murakami, Chikako, Yuki Mizutani, Akemi Ryu, Eiji Naru, Takashi Teramura, Yuta Homma, and Mitsunori Fukuda. 2020. "A Novel Method for Visualizing Melanosome and Melanin Distribution in Human Skin Tissues" International Journal of Molecular Sciences 21, no. 22: 8514. https://doi.org/10.3390/ijms21228514

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