Isolinderalactone Induces Cell Death via Mitochondrial Superoxide- and STAT3-Mediated Pathways in Human Ovarian Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. ILL Induced Apoptosis in Human OC Cells
2.2. ILL Activated the Caspase Cascade and Generated mtSO during Apoptosis
2.3. ILL Down-Regulated the Expression Levels of Proapoptotic Bcl-2 Family Proteins and SOD2
2.4. ILL Suppressed Activation of the JAK/STAT3 Signaling Pathway
2.5. Specific Scavenging of mtSO Reverses ILL-Induced Apoptosis
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Cell Culture
4.2. Chemicals and Antibodies
4.3. Cell Viability Assay
4.4. Measurement of Cell Death and Assessment of the Cell Cycle Phase after ILL Treatment
4.5. Measurements of ROS and mtSO Contents, and MMP
4.6. Immunoblot Analysis
4.7. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Rajina, S.; Kim, W.J.; Shim, J.-H.; Chun, K.-S.; Joo, S.H.; Shin, H.K.; Lee, S.-Y.; Choi, J.-S. Isolinderalactone Induces Cell Death via Mitochondrial Superoxide- and STAT3-Mediated Pathways in Human Ovarian Cancer Cells. Int. J. Mol. Sci. 2020, 21, 7530. https://doi.org/10.3390/ijms21207530
Rajina S, Kim WJ, Shim J-H, Chun K-S, Joo SH, Shin HK, Lee S-Y, Choi J-S. Isolinderalactone Induces Cell Death via Mitochondrial Superoxide- and STAT3-Mediated Pathways in Human Ovarian Cancer Cells. International Journal of Molecular Sciences. 2020; 21(20):7530. https://doi.org/10.3390/ijms21207530
Chicago/Turabian StyleRajina, Shakya, Woo Jean Kim, Jung-Hyun Shim, Kyung-Soo Chun, Sang Hoon Joo, Hwa Kyoung Shin, Seo-Yeon Lee, and Joon-Seok Choi. 2020. "Isolinderalactone Induces Cell Death via Mitochondrial Superoxide- and STAT3-Mediated Pathways in Human Ovarian Cancer Cells" International Journal of Molecular Sciences 21, no. 20: 7530. https://doi.org/10.3390/ijms21207530