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Open AccessArticle

Platelet-Rich Fibrin Facilitates One-Stage Cartilage Repair by Promoting Chondrocytes Viability, Migration, and Matrix Synthesis

by Chin-Chean Wong 1,2,3,4,†, Keng-Liang Ou 5,6,7,8,9,†, Yun-Ho Lin 10,11, Ming-Fang Lin 12,13, Tsung-Lin Yang 14,15,16,17, Chih-Hwa Chen 1,3,18,19 and Wing P. Chan 13,20,21,*
1
Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan
2
Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
3
Research Center of Biomedical Devices, Taipei Medical University, Taipei 11031, Taiwan
4
International Ph.D. Program for Cell Therapy and Regenerative Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
5
Department of Dentistry, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan
6
Department of Oral Hygiene Care, Ching Kuo Institute of Management and Health, Keelung 20301, Taiwan
7
Department of Dentistry, Taipei Medical University Hospital, Taipei 11031, Taiwan
8
School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan
9
3D Global Biotech Inc., New Taipei City 22175, Taiwan
10
Division of Oral Pathology, Department of Dentistry, Taipei Medical University Hospital, Taipei 11031, Taiwan
11
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan
12
Department of Medical Imaging and Radiological Technology, Yuanpei University, Hsinchu 30015, Taiwan
13
Department of Radiology, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan
14
Department of Otolaryngology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei 10051, Taiwan
15
Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei 10051, Taiwan
16
Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei 10051, Taiwan
17
Department of Medical Research, National Taiwan University Hospital, Taipei 10051, Taiwan
18
School of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan
19
School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
20
Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
21
Medical Innovation Development Center, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(2), 577; https://doi.org/10.3390/ijms21020577
Received: 20 December 2019 / Revised: 9 January 2020 / Accepted: 13 January 2020 / Published: 16 January 2020
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
The main aim of this study is to develop a one-stage method to combine platelet-rich fibrin (PRF) and autologous cartilage autografts for porcine articular cartilage repair. The porcine chondrocytes were treated with different concentrations of PRF-conditioned media and were evaluated for their cell viability and extracellular glycosaminoglycan (GAG) synthesis during six day cultivation. The chemotactic effects of PRF on chondrocytes on undigested cartilage autografts were revealed in explant cultures. For the in vivo part, porcine chondral defects were created at the medial femoral condyles of which were (1) left untreated, (2) implanted with PRF combined with hand-diced cartilage grafts, or (3) implanted with PRF combined with device-diced cartilage grafts. After six months, gross grades, histological, and immunohistochemical analyses were compared. The results showed that PRF promotes the viability and GAG expression of the cultured chondrocytes. Additionally, the PRF-conditioned media induce significant cellular migration and outgrowth of chondrocytes from undigested cartilage grafts. In the in vivo study, gross grading and histological scores showed significantly better outcomes in the treatment groups as compared with controls. Moreover, both treatment groups showed significantly more type II collagen staining and minimal type I collagen staining as compared with controls, indicating more hyaline-like cartilage and less fibrous tissue. In conclusion, PRF enhances the viability, differentiation, and migration of chondrocytes, thus, showing an appealing capacity for cartilage repair. The data altogether provide evidences to confirm the feasibility of a one-stage, culture-free method of combining PRF and cartilage autografts for repairing articular cartilage defects. From translational standpoints, these advantages benefit clinical applications by simplifying and potentiating the efficacy of cartilage autograft transplants. View Full-Text
Keywords: autografts; cartilage; chondrocytes; knee; platelet-rich fibrin (PRF) autografts; cartilage; chondrocytes; knee; platelet-rich fibrin (PRF)
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MDPI and ACS Style

Wong, C.-C.; Ou, K.-L.; Lin, Y.-H.; Lin, M.-F.; Yang, T.-L.; Chen, C.-H.; Chan, W.P. Platelet-Rich Fibrin Facilitates One-Stage Cartilage Repair by Promoting Chondrocytes Viability, Migration, and Matrix Synthesis. Int. J. Mol. Sci. 2020, 21, 577.

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