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Open AccessArticle

Expression of Thioredoxin/Thioredoxin Reductase System Genes in Aphid-Challenged Maize Seedlings

1
Institute of Biological Sciences, Faculty of Exact and Natural Sciences, Siedlce University of Natural Sciences and Humanities, 14 Prusa St., 08-110 Siedlce, Poland
2
Department of Plant Protection, Faculty of Horticulture and Landscape Architecture, University of Life Sciences in Lublin, 7 Leszczyńskiego St., 20-069 Lublin, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6296; https://doi.org/10.3390/ijms21176296
Received: 31 July 2020 / Revised: 27 August 2020 / Accepted: 29 August 2020 / Published: 31 August 2020
(This article belongs to the Special Issue Plant Innate Immunity 4.0)
Thioredoxins (Trxs) and thioredoxin reductases (TrxRs) encompass a highly complex network involved in sustaining thiol-based redox homeostasis in plant tissues. The purpose of the study was to gain a new insight into transcriptional reprogramming of the several genes involved in functioning of Trx/TrxR system in maize (Zea mays L.) seedlings, exposed to the bird cherry-oat aphid (Rhopalosiphum padi L.) or the rose-grass aphid (Metopolophium dirhodum Walk.) infestation. The biotests were performed on two maize genotypes (susceptible Złota Karłowa and relatively resistant Waza). The application of real-time qRT-PCR technique allowed to identify a molecular mechanism triggered in more resistant maize plants, linked to upregulation of thioredoxins-encoding genes (Trx-f, Trx-h, Trx-m, Trx-x) and thioredoxin reductase genes (Ftr1, Trxr2). Significant enhancement of TrxR activity in aphid-infested Waza seedlings was also demonstrated. Furthermore, we used an electrical penetration graph (EPG) recordings of M. dirhodum stylet activities in seedlings of the two studied maize varieties. Duration of phloem phase (E1 and E2 models) of rose-grass aphids was about three times longer while feeding in Waza plants, compared to Złota Karłowa cv. The role of activation of Trx/TrxR system in maintaining redox balance and counteracting oxidative-induced damages of macromolecules in aphid-stressed maize plants is discussed. View Full-Text
Keywords: maize; Rhopalosiphum padi; Metopolophium dirhodum; gene expression; thioredoxin; thioredoxin reductase maize; Rhopalosiphum padi; Metopolophium dirhodum; gene expression; thioredoxin; thioredoxin reductase
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MDPI and ACS Style

Sytykiewicz, H.; Łukasik, I.; Goławska, S.; Sprawka, I.; Goławski, A.; Sławianowska, J.; Kmieć, K. Expression of Thioredoxin/Thioredoxin Reductase System Genes in Aphid-Challenged Maize Seedlings. Int. J. Mol. Sci. 2020, 21, 6296. https://doi.org/10.3390/ijms21176296

AMA Style

Sytykiewicz H, Łukasik I, Goławska S, Sprawka I, Goławski A, Sławianowska J, Kmieć K. Expression of Thioredoxin/Thioredoxin Reductase System Genes in Aphid-Challenged Maize Seedlings. International Journal of Molecular Sciences. 2020; 21(17):6296. https://doi.org/10.3390/ijms21176296

Chicago/Turabian Style

Sytykiewicz, Hubert; Łukasik, Iwona; Goławska, Sylwia; Sprawka, Iwona; Goławski, Artur; Sławianowska, Julia; Kmieć, Katarzyna. 2020. "Expression of Thioredoxin/Thioredoxin Reductase System Genes in Aphid-Challenged Maize Seedlings" Int. J. Mol. Sci. 21, no. 17: 6296. https://doi.org/10.3390/ijms21176296

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