Next Article in Journal
Fock-Space Coupled Cluster Theory: Systematic Study of Partial Fourth Order Triples Schemes for Ionization Potential and Comparison with Bondonic Formalism
Next Article in Special Issue
The Penta-EF-Hand ALG-2 Protein Interacts with the Cytosolic Domain of the SOCE Regulator SARAF and Interferes with Ubiquitination
Previous Article in Journal
The Role of Amino Acids in Neurotransmission and Fluorescent Tools for Their Detection
Previous Article in Special Issue
ASGR1 and Its Enigmatic Relative, CLEC10A
Open AccessArticle

Knockout of stim2a Increases Calcium Oscillations in Neurons and Induces Hyperactive-Like Phenotype in Zebrafish Larvae

International Institute of Molecular and Cell Biology in Warsaw, Ks. Trojdena 4, 02-109 Warsaw, Poland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
PhD students at the School of Molecular Medicine, Warsaw Medical University.
Int. J. Mol. Sci. 2020, 21(17), 6198; https://doi.org/10.3390/ijms21176198
Received: 3 June 2020 / Revised: 20 August 2020 / Accepted: 24 August 2020 / Published: 27 August 2020
(This article belongs to the Special Issue Calcium-Binding Proteins and Cell Signaling 2.0)
Stromal interaction molecule (STIM) proteins play a crucial role in store-operated calcium entry (SOCE) as endoplasmic reticulum Ca2+ sensors. In neurons, STIM2 was shown to have distinct functions from STIM1. However, its role in brain activity and behavior was not fully elucidated. The present study analyzed behavior in zebrafish (Danio rerio) that lacked stim2a. The mutant animals had no morphological abnormalities and were fertile. RNA-sequencing revealed alterations of the expression of transcription factor genes and several members of the calcium toolkit. Neuronal Ca2+ activity was measured in vivo in neurons that expressed the GCaMP5G sensor. Optic tectum neurons in stim2a−/− fish had more frequent Ca2+ signal oscillations compared with neurons in wildtype (WT) fish. We detected an increase in activity during the visual–motor response test, an increase in thigmotaxis in the open field test, and the disruption of phototaxis in the dark/light preference test in stim2a−/− mutants compared with WT. Both groups of animals reacted to glutamate and pentylenetetrazol with an increase in activity during the visual–motor response test, with no major differences between groups. Altogether, our results suggest that the hyperactive-like phenotype of stim2a−/− mutant zebrafish is caused by the dysregulation of Ca2+ homeostasis and signaling. View Full-Text
Keywords: SOCE; Stim2a; calcium toolkit; zebrafish; behavioral tests; hyperactivity; glutamate; PTZ SOCE; Stim2a; calcium toolkit; zebrafish; behavioral tests; hyperactivity; glutamate; PTZ
Show Figures

Figure 1

MDPI and ACS Style

Gupta, R.K.; Wasilewska, I.; Palchevska, O.; Kuźnicki, J. Knockout of stim2a Increases Calcium Oscillations in Neurons and Induces Hyperactive-Like Phenotype in Zebrafish Larvae. Int. J. Mol. Sci. 2020, 21, 6198. https://doi.org/10.3390/ijms21176198

AMA Style

Gupta RK, Wasilewska I, Palchevska O, Kuźnicki J. Knockout of stim2a Increases Calcium Oscillations in Neurons and Induces Hyperactive-Like Phenotype in Zebrafish Larvae. International Journal of Molecular Sciences. 2020; 21(17):6198. https://doi.org/10.3390/ijms21176198

Chicago/Turabian Style

Gupta, Rishikesh K.; Wasilewska, Iga; Palchevska, Oksana; Kuźnicki, Jacek. 2020. "Knockout of stim2a Increases Calcium Oscillations in Neurons and Induces Hyperactive-Like Phenotype in Zebrafish Larvae" Int. J. Mol. Sci. 21, no. 17: 6198. https://doi.org/10.3390/ijms21176198

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop