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Open AccessArticle

Tuning Adipogenic Differentiation in ADSCs by Metformin and Vitamin D: Involvement of miRNAs

1
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
2
Department of Medical, Surgical and Experimental Sciences, General Surgery Unit 2 “Clinica Chirurgica”, University of Sassari, Viale San Pietro 8, 07100 Sassari, Italy
3
Laboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems-Eldor Lab, Innovation Accelerator, Consiglio Nazionale delle Ricerche, 40129 Bologna, Italy
4
Department of Biomedical Sciences, Center for Developmental Biology and Reprogramming (CEDEBIOR), University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy
5
Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche (CNR), 09042 Monserrato, Cagliari, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6181; https://doi.org/10.3390/ijms21176181
Received: 7 August 2020 / Revised: 23 August 2020 / Accepted: 26 August 2020 / Published: 27 August 2020
(This article belongs to the Special Issue MicroRNAs and Stem Cells 2.0)
Fat tissue represents an important source of adipose-derived stem cells (ADSCs), which can differentiate towards several phenotypes under certain stimuli. Definite molecules as vitamin D are able to influence stem cell fate, acting on the expression of specific genes. In addition, miRNAs are important modulating factors in obesity and numerous diseases. We previously identified specific conditioned media able to commit stem cells towards defined cellular phenotypes. In the present paper, we aimed at evaluating the role of metformin on ADSCs differentiation. In particular, ADSCs were cultured in a specific adipogenic conditioned medium (MD), in the presence of metformin, alone or in combination with vitamin D. Our results showed that the combination of the two compounds is able to counteract the appearance of an adipogenic phenotype, indicating a feedforward regulation on vitamin D metabolism by metformin, acting on CYP27B1 and CYP3A4. We then evaluated the role of specific epigenetic modulating genes and miRNAs in controlling stem cell adipogenesis. The combination of the two molecules was able to influence stem cell fate, by modulating the adipogenic phenotype, suggesting their possible application in clinical practice in counteracting uncontrolled lipogenesis and obesity-related diseases. View Full-Text
Keywords: stem cells; miRNA; cellular mechanisms; gene expression; epigenetic; adipogenesis; conditioned media stem cells; miRNA; cellular mechanisms; gene expression; epigenetic; adipogenesis; conditioned media
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MDPI and ACS Style

Cruciani, S.; Garroni, G.; Balzano, F.; Pala, R.; Bellu, E.; Cossu, M.L.; Ginesu, G.C.; Ventura, C.; Maioli, M. Tuning Adipogenic Differentiation in ADSCs by Metformin and Vitamin D: Involvement of miRNAs. Int. J. Mol. Sci. 2020, 21, 6181. https://doi.org/10.3390/ijms21176181

AMA Style

Cruciani S, Garroni G, Balzano F, Pala R, Bellu E, Cossu ML, Ginesu GC, Ventura C, Maioli M. Tuning Adipogenic Differentiation in ADSCs by Metformin and Vitamin D: Involvement of miRNAs. International Journal of Molecular Sciences. 2020; 21(17):6181. https://doi.org/10.3390/ijms21176181

Chicago/Turabian Style

Cruciani, Sara; Garroni, Giuseppe; Balzano, Francesca; Pala, Renzo; Bellu, Emanuela; Cossu, Maria L.; Ginesu, Giorgio C.; Ventura, Carlo; Maioli, Margherita. 2020. "Tuning Adipogenic Differentiation in ADSCs by Metformin and Vitamin D: Involvement of miRNAs" Int. J. Mol. Sci. 21, no. 17: 6181. https://doi.org/10.3390/ijms21176181

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