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Sperm Motility of Mice under Simulated Microgravity and Hypergravity
Article

Drosophila melanogaster Sperm under Simulated Microgravity and a Hypomagnetic Field: Motility and Cell Respiration

1
Cell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, 123007 Moscow, Russia
2
Department of Medical and Biological Physics, I. M. Sechenov First Moscow State Medical University, 8-2 Trubetskaya St., 119991 Moscow, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 5985; https://doi.org/10.3390/ijms21175985
Received: 31 July 2020 / Revised: 11 August 2020 / Accepted: 18 August 2020 / Published: 20 August 2020
(This article belongs to the Special Issue Microgravity and Space Medicine)
The role of the Earth’s gravitational and magnetic fields in the evolution and maintenance of normal processes of various animal species remains unclear. The aim of this work was to determine the effect of simulated microgravity and hypomagnetic conditions for 1, 3, and 6 h on the sperm motility of the fruit fly Drosophila melanogaster. In addition to the usual diet, the groups were administered oral essential phospholipids at a dosage of 500 mg/kg in medium. The speed of the sperm tails was determined by video recording and analysis of the obtained video files, protein content by western blotting, and cell respiration by polarography. The results indicated an increase in the speed of movement of the sperm tails after 6 h in simulated microgravity. The levels of proteins that form the axoneme of the sperm tail did not change, but cellular respiration was altered. A similar effect occurred with the administration of essential phospholipids. These results may be due to a change in the level of phosphorylation of motor proteins. Exposure to hypomagnetic conditions led to a decrease in motility after 6 h against a background of a decrease in the rate of cellular respiration due to complex I of the respiratory chain. This effect was not observed in the flies that received essential phospholipids. However, after 1 h under hypomagnetic conditions, the rate of cellular respiration also increased due to complex I, including that in the sperm of flies receiving essential phospholipids. View Full-Text
Keywords: sperm; hypomagnetic field; simulated microgravity; motility; cell respiration sperm; hypomagnetic field; simulated microgravity; motility; cell respiration
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MDPI and ACS Style

Ogneva, I.V.; Usik, M.A.; Burtseva, M.V.; Biryukov, N.S.; Zhdankina, Y.S.; Sychev, V.N.; Orlov, O.I. Drosophila melanogaster Sperm under Simulated Microgravity and a Hypomagnetic Field: Motility and Cell Respiration. Int. J. Mol. Sci. 2020, 21, 5985. https://doi.org/10.3390/ijms21175985

AMA Style

Ogneva IV, Usik MA, Burtseva MV, Biryukov NS, Zhdankina YS, Sychev VN, Orlov OI. Drosophila melanogaster Sperm under Simulated Microgravity and a Hypomagnetic Field: Motility and Cell Respiration. International Journal of Molecular Sciences. 2020; 21(17):5985. https://doi.org/10.3390/ijms21175985

Chicago/Turabian Style

Ogneva, Irina V., Maria A. Usik, Maria V. Burtseva, Nikolay S. Biryukov, Yuliya S. Zhdankina, Vladimir N. Sychev, and Oleg I. Orlov 2020. "Drosophila melanogaster Sperm under Simulated Microgravity and a Hypomagnetic Field: Motility and Cell Respiration" International Journal of Molecular Sciences 21, no. 17: 5985. https://doi.org/10.3390/ijms21175985

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