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Open AccessArticle

An EPR Study on the Interaction between the Cu(I) Metal Binding Domains of ATP7B and the Atox1 Metallochaperone

Department of Chemistry, Faculty of Exact Sciences, Bar Ilan University, Ramat-Gan 5290002, Israel
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(15), 5536; https://doi.org/10.3390/ijms21155536
Received: 19 July 2020 / Revised: 29 July 2020 / Accepted: 30 July 2020 / Published: 2 August 2020
Copper’s essentiality and toxicity mean it requires a sophisticated regulation system for its acquisition, cellular distribution and excretion, which until now has remained elusive. Herein, we applied continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) spectroscopy in solution to resolve the copper trafficking mechanism in humans, by considering the route travelled by Cu(I) from the metallochaperone Atox1 to the metal binding domains of ATP7B. Our study revealed that Cu(I) is most likely mediated by the binding of the Atox1 monomer to metal binding domain 1 (MBD1) and MBD4 of ATP7B in the final part of its extraction pathway, while the other MBDs mediate this interaction and participate in copper transfer between the various MBDs to the ATP7B membrane domain. This research also proposes that MBD1-3 and MBD4-6 act as two independent units. View Full-Text
Keywords: copper metabolism; metal binding domains; Atox1; ATP7B; EPR; DEER copper metabolism; metal binding domains; Atox1; ATP7B; EPR; DEER
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MDPI and ACS Style

Zaccak, M.; Qasem, Z.; Gevorkyan-Airapetov, L.; Ruthstein, S. An EPR Study on the Interaction between the Cu(I) Metal Binding Domains of ATP7B and the Atox1 Metallochaperone. Int. J. Mol. Sci. 2020, 21, 5536. https://doi.org/10.3390/ijms21155536

AMA Style

Zaccak M, Qasem Z, Gevorkyan-Airapetov L, Ruthstein S. An EPR Study on the Interaction between the Cu(I) Metal Binding Domains of ATP7B and the Atox1 Metallochaperone. International Journal of Molecular Sciences. 2020; 21(15):5536. https://doi.org/10.3390/ijms21155536

Chicago/Turabian Style

Zaccak, Michael; Qasem, Zena; Gevorkyan-Airapetov, Lada; Ruthstein, Sharon. 2020. "An EPR Study on the Interaction between the Cu(I) Metal Binding Domains of ATP7B and the Atox1 Metallochaperone" Int. J. Mol. Sci. 21, no. 15: 5536. https://doi.org/10.3390/ijms21155536

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