Next Article in Journal
The Roles of GRKs in Hemostasis and Thrombosis
Next Article in Special Issue
Genetic and Methylome Variation in Turkish Brachypodium Distachyon Accessions Differentiate Two Geographically Distinct Subpopulations
Previous Article in Journal
The Role of CDKs and CDKIs in Murine Development
Previous Article in Special Issue
Silencing of TaCKX1 Mediates Expression of Other TaCKX Genes to Increase Yield Parameters in Wheat
Open AccessArticle

Identification and Expression Analysis of Hormone Biosynthetic and Metabolism Genes in the 2OGD Family for Identifying Genes That May Be Involved in Tomato Fruit Ripening

1
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
2
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
3
Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(15), 5344; https://doi.org/10.3390/ijms21155344
Received: 30 June 2020 / Revised: 20 July 2020 / Accepted: 23 July 2020 / Published: 28 July 2020
(This article belongs to the Special Issue Plant Genomics 2019)
Phytohormones play important roles in modulating tomato fruit development and ripening. The 2-oxoglutarate-dependent dioxygenase (2OGD) superfamily containing several subfamilies involved in hormone biosynthesis and metabolism. In this study, we aimed to identify hormone biosynthesis and metabolism-related to 2OGD proteins in tomato and explored their roles in fruit development and ripening. We identified nine 2OGD protein subfamilies involved in hormone biosynthesis and metabolism, including the gibberellin (GA) biosynthetic protein families GA20ox and GA3ox, GA degradation protein families C19-GA2ox and C20-GA2ox, ethylene biosynthetic protein family ACO, auxin degradation protein family DAO, jasmonate hydroxylation protein family JOX, salicylic acid degradation protein family DMR6, and strigolactone biosynthetic protein family LBO. These genes were differentially expressed in different tomato organs. The GA degradation gene SlGA2ox2, and the auxin degradation gene SlDAO1, showed significantly increased expression from the mature-green to the breaker stage during tomato fruit ripening, accompanied by decreased endogenous GA and auxin, indicating that SlGA2ox2 and SlDAO1 were responsible for the reduced GA and auxin concentrations. Additionally, exogenous gibberellin 3 (GA3) and indole-3-acetic acid (IAA) treatment of mature-green fruits delayed fruit ripening and increased the expression of SlGA2ox2 and SlDAO1, respectively. Therefore, SlGA2ox2 and SlDAO1 are implicated in the degradation of GAs and auxin during tomato fruit ripening. View Full-Text
Keywords: genome-wide identification; expression analysis; 2OGD family; hormone biosynthetic and metabolism genes; tomato fruit ripening genome-wide identification; expression analysis; 2OGD family; hormone biosynthetic and metabolism genes; tomato fruit ripening
Show Figures

Figure 1

MDPI and ACS Style

Ding, Q.; Wang, F.; Xue, J.; Yang, X.; Fan, J.; Chen, H.; Li, Y.; Wu, H. Identification and Expression Analysis of Hormone Biosynthetic and Metabolism Genes in the 2OGD Family for Identifying Genes That May Be Involved in Tomato Fruit Ripening. Int. J. Mol. Sci. 2020, 21, 5344. https://doi.org/10.3390/ijms21155344

AMA Style

Ding Q, Wang F, Xue J, Yang X, Fan J, Chen H, Li Y, Wu H. Identification and Expression Analysis of Hormone Biosynthetic and Metabolism Genes in the 2OGD Family for Identifying Genes That May Be Involved in Tomato Fruit Ripening. International Journal of Molecular Sciences. 2020; 21(15):5344. https://doi.org/10.3390/ijms21155344

Chicago/Turabian Style

Ding, Qiangqiang; Wang, Feng; Xue, Juan; Yang, Xinxin; Fan, Junmiao; Chen, Hong; Li, Yi; Wu, Han. 2020. "Identification and Expression Analysis of Hormone Biosynthetic and Metabolism Genes in the 2OGD Family for Identifying Genes That May Be Involved in Tomato Fruit Ripening" Int. J. Mol. Sci. 21, no. 15: 5344. https://doi.org/10.3390/ijms21155344

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop