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Article

Anti-Inflammatory Potential of n-3 Polyunsaturated Fatty Acids Enriched Hen Eggs Consumption in Improving Microvascular Endothelial Function of Healthy Individuals—Clinical Trial

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Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, HR-31000 Osijek, Croatia
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Scientific Center of Excellence for Personalized Health Care, Josip Juraj Strossmayer University of Osijek, Trg Svetog Trojstva 3, Hr-31000 Osijek, Croatia
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Department of Pathophysiology, Physiology and Immunology, Faculty of Dental Medicine and Health Osijek, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10E, HR-31000 Osijek, Croatia
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Department of Dermatology and Venereology, Osijek University Hospital, J. Huttlera 4, HR-31000 Osijek, Croatia
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Department of Internal Medicine, Vukovar General Hospital, Županijska ulica 35, HR-32000 Vukovar, Croatia
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Department for Cardiovascular Disease, Osijek University Hospital, J. Huttlera 4, HR-31000 Osijek, Croatia
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Department of Animal Production and Biotechnology, Faculty of Agrobiotechnical Sciences, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, HR-31000 Osijek, Croatia
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Department of Clinical Laboratory Diagnostics, Osijek University Hospital, J. Huttlera 4, HR-31000 Osijek, Croatia
*
Author to whom correspondence should be addressed.
These authors contribute equally to the article.
Int. J. Mol. Sci. 2020, 21(11), 4149; https://doi.org/10.3390/ijms21114149
Received: 19 May 2020 / Revised: 1 June 2020 / Accepted: 9 June 2020 / Published: 10 June 2020
(This article belongs to the Section Bioactives and Nutraceuticals)
The effects of consumption of n-3 polyunsaturated fatty acids (n-3 PUFAs) enriched hen eggs on endothelium-dependent and endothelium-independent vasodilation in microcirculation, and on endothelial activation and inflammation were determined in young healthy individuals. Control group (N = 21) ate three regular hen eggs/daily (249 mg n-3 PUFAs/day), and n-3 PUFAs group (N = 19) ate three n-3 PUFAs enriched hen eggs/daily (1053 g n-3 PUFAs/day) for 3 weeks. Skin microvascular blood flow in response to iontophoresis of acetylcholine (AChID; endothelium-dependent) and sodium nitroprusside (SNPID; endothelium-independent) was assessed by laser Doppler flowmetry. Blood pressure (BP), body composition, body fluid status, serum lipid and free fatty acids profile, and inflammatory and endothelial activation markers were measured before and after respective dietary protocol. Results: Serum n-3 PUFAs concentration significantly increased, AChID significantly improved, and SNPID remained unchanged in n-3 PUFAs group, while none was changed in Control group. Interferon-γ (pro-inflammatory) significantly decreased and interleukin-10 (anti-inflammatory) significantly increased in n-3 PUFAs. BP, fat free mass, and total body water significantly decreased, while fat mass, interleukin-17A (pro-inflammatory), interleukin-10 and vascular endothelial growth factor A significantly increased in the Control group. Other measured parameters remained unchanged in both groups. Favorable anti-inflammatory properties of n-3 PUFAs consumption potentially contribute to the improvement of microvascular endothelium-dependent vasodilation in healthy individuals. View Full-Text
Keywords: n-3 polyunsaturated fatty acids; omega-3 polyunsaturated fatty acids; endothelium; microvascular; inflammation; laser Doppler flowmetry; cytokines n-3 polyunsaturated fatty acids; omega-3 polyunsaturated fatty acids; endothelium; microvascular; inflammation; laser Doppler flowmetry; cytokines
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MDPI and ACS Style

Stupin, A.; Mihalj, M.; Kolobarić, N.; Šušnjara, P.; Kolar, L.; Mihaljević, Z.; Matić, A.; Stupin, M.; Jukić, I.; Kralik, Z.; Grčević, M.; Kralik, G.; Šerić, V.; Drenjančević, I. Anti-Inflammatory Potential of n-3 Polyunsaturated Fatty Acids Enriched Hen Eggs Consumption in Improving Microvascular Endothelial Function of Healthy Individuals—Clinical Trial. Int. J. Mol. Sci. 2020, 21, 4149. https://doi.org/10.3390/ijms21114149

AMA Style

Stupin A, Mihalj M, Kolobarić N, Šušnjara P, Kolar L, Mihaljević Z, Matić A, Stupin M, Jukić I, Kralik Z, Grčević M, Kralik G, Šerić V, Drenjančević I. Anti-Inflammatory Potential of n-3 Polyunsaturated Fatty Acids Enriched Hen Eggs Consumption in Improving Microvascular Endothelial Function of Healthy Individuals—Clinical Trial. International Journal of Molecular Sciences. 2020; 21(11):4149. https://doi.org/10.3390/ijms21114149

Chicago/Turabian Style

Stupin, Ana, Martina Mihalj, Nikolina Kolobarić, Petar Šušnjara, Luka Kolar, Zrinka Mihaljević, Anita Matić, Marko Stupin, Ivana Jukić, Zlata Kralik, Manuela Grčević, Gordana Kralik, Vatroslav Šerić, and Ines Drenjančević. 2020. "Anti-Inflammatory Potential of n-3 Polyunsaturated Fatty Acids Enriched Hen Eggs Consumption in Improving Microvascular Endothelial Function of Healthy Individuals—Clinical Trial" International Journal of Molecular Sciences 21, no. 11: 4149. https://doi.org/10.3390/ijms21114149

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