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Open AccessArticle

Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches

School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk 28644, Korea
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea
Department of Earth Sciences, Paleobiology, Uppsala University, Villavägen 16, 75236 Uppsala, Sweden
Department of Life Sciences, Chung-Ang University, Seoul 156-756, Korea
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(16), 3994;
Received: 9 July 2019 / Revised: 7 August 2019 / Accepted: 14 August 2019 / Published: 16 August 2019
(This article belongs to the Special Issue Regulation of Gene Expression During Embryonic Development)
Antistasin, which was originally discovered in the salivary glands of the Mexican leech Haementeria officinalis, was newly isolated from Helobdella austinensis. To confirm the temporal expression of antistasin during embryogenesis, we carried out semi-quantitative RT-PCR. Hau-antistasin1 was uniquely expressed at stage 4 of the cleavage and was strongly expressed in the late stages of organogenesis, as were other antistasin members. In order to confirm the spatial expression of antistasin, we performed fluorescence in situ hybridization in the late stages of organogenesis. The expression of each antistasin in the proboscis showed a similar pattern and varied in expression in the body. In addition, the spatial expression of antistasin orthologs in different leeches showed the possibility of different function across leech species. Hau-antistasin1 was expressed in the same region as hedgehog, which is a known mediator of signal transduction pathway. Hau-antistasin1 is probably a downstream target of Hedgehog signaling, involved in segment polarity signal pathway. View Full-Text
Keywords: Antistasin; Helobdella; in situ hybridization; expression; hedgehog signaling Antistasin; Helobdella; in situ hybridization; expression; hedgehog signaling
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MDPI and ACS Style

Kwak, H.-J.; Park, J.-S.; Medina Jiménez, B.I.; Park, S.C.; Cho, S.-J. Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches. Int. J. Mol. Sci. 2019, 20, 3994.

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