Next Article in Journal
Antioxidant and Cytoprotective Effects of (−)-Epigallocatechin-3-(3″-O-methyl) Gallate
Previous Article in Journal
Topographical Visualization of the Reciprocal Projection between the Medial Septum and the Hippocampus in the 5XFAD Mouse Model of Alzheimer’s Disease
Open AccessArticle

Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches

1
School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk 28644, Korea
2
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea
3
Department of Earth Sciences, Paleobiology, Uppsala University, Villavägen 16, 75236 Uppsala, Sweden
4
Department of Life Sciences, Chung-Ang University, Seoul 156-756, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(16), 3994; https://doi.org/10.3390/ijms20163994
Received: 9 July 2019 / Revised: 7 August 2019 / Accepted: 14 August 2019 / Published: 16 August 2019
(This article belongs to the Special Issue Regulation of Gene Expression During Embryonic Development)
  |  
PDF [2547 KB, uploaded 16 August 2019]
  |  

Abstract

Antistasin, which was originally discovered in the salivary glands of the Mexican leech Haementeria officinalis, was newly isolated from Helobdella austinensis. To confirm the temporal expression of antistasin during embryogenesis, we carried out semi-quantitative RT-PCR. Hau-antistasin1 was uniquely expressed at stage 4 of the cleavage and was strongly expressed in the late stages of organogenesis, as were other antistasin members. In order to confirm the spatial expression of antistasin, we performed fluorescence in situ hybridization in the late stages of organogenesis. The expression of each antistasin in the proboscis showed a similar pattern and varied in expression in the body. In addition, the spatial expression of antistasin orthologs in different leeches showed the possibility of different function across leech species. Hau-antistasin1 was expressed in the same region as hedgehog, which is a known mediator of signal transduction pathway. Hau-antistasin1 is probably a downstream target of Hedgehog signaling, involved in segment polarity signal pathway. View Full-Text
Keywords: Antistasin; Helobdella; in situ hybridization; expression; hedgehog signaling Antistasin; Helobdella; in situ hybridization; expression; hedgehog signaling
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Kwak, H.-J.; Park, J.-S.; Medina Jiménez, B.I.; Park, S.C.; Cho, S.-J. Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches. Int. J. Mol. Sci. 2019, 20, 3994.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top