Zr-89 Immuno-PET Targeting Ectopic ATP Synthase Enables In-Vivo Imaging of Tumor Angiogenesis
AbstractIn this study, we synthesized a Zr-89-labeled anti-adenosine triphosphate synthase monoclonal antibody (ATPS mAb) for applications in immuno-positron emission tomography (PET) and evaluated its feasibility for angiogenesis imaging. The cellular uptake of Zr-89 ATPS mAb was measured after treatment of cancer cell lines in vitro, and its biodistribution was evaluated at 4, 24 and 48 h in vivo in mice bearing xenografts. PET images were acquired at 4, 24, 48, and 96 h after Zr-89 ATPS mAb administration. Tumor angiogenesis was analyzed using anti-CD31 immunofluorescence staining. The cellular uptake of Zr-89 ATPS mAb increased over time in MDA-MB-231 breast cancer cells but did not increase in PC3 prostate cancer cells. The tumor uptake of Zr-89 ATPS mAb at 24 h was 9.4 ± 0.9% ID/g for MDA-Mb-231 cells and was 3.8 ± 0.6% ID/g for PC3 cells (p = 0.004). Zr-89 ATPS mAb uptake in MDA-MB-231 xenografts was inhibited by the administration of cold ATPS mAb (4.4 ± 0.5% ID/g, p = 0.011). Zr-89 ATPS mAb uptake could be visualized by PET for up to 96 h in MDA-MB-231 tumors. In contrast, there was no distinct tumor uptake detected by PET in the PC3 xenograft model. CD31-positive tumor vessels were abundant in MDA-MB-231 tumors, whereas they were scarcely detected in PC3 tumors. In conclusion, ATPS mAb was successfully labeled with Zr-89, which could be used for immuno-PET imaging targeting tumor angiogenesis. View Full-Text
- Supplementary File 1:
ZIP-Document (ZIP, 592 KB)
Share & Cite This Article
Park, B.-N.; Kim, G.-H.; Ko, S.-A.; Shin, G.-H.; Lee, S.-J.; An, Y.-S.; Yoon, J.-K. Zr-89 Immuno-PET Targeting Ectopic ATP Synthase Enables In-Vivo Imaging of Tumor Angiogenesis. Int. J. Mol. Sci. 2019, 20, 3928.
Park B-N, Kim G-H, Ko S-A, Shin G-H, Lee S-J, An Y-S, Yoon J-K. Zr-89 Immuno-PET Targeting Ectopic ATP Synthase Enables In-Vivo Imaging of Tumor Angiogenesis. International Journal of Molecular Sciences. 2019; 20(16):3928.Chicago/Turabian Style
Park, Bok-Nam; Kim, Ga-Hee; Ko, Seung-A; Shin, Ga-Hee; Lee, Su-Jin; An, Young-Sil; Yoon, Joon-Kee. 2019. "Zr-89 Immuno-PET Targeting Ectopic ATP Synthase Enables In-Vivo Imaging of Tumor Angiogenesis." Int. J. Mol. Sci. 20, no. 16: 3928.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.