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Int. J. Mol. Sci. 2018, 19(11), 3506; https://doi.org/10.3390/ijms19113506

Study on Cardiotoxicity and Mechanism of “Fuzi” Extracts Based on Metabonomics

1
Department of Pharmacology, Basic Medical College, Anhui Medical University, Hefei 230032, China
2
Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine, Beijing 100850, China
Both authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 19 September 2018 / Revised: 26 October 2018 / Accepted: 5 November 2018 / Published: 7 November 2018
(This article belongs to the Special Issue Traditional Medicine – Unraveling Its Molecular Mechanism, Volume 2)
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Abstract

To investigate the toxicity of water and ethanol “Fuzi” (FZ) extracts and to explore the toxicity mechanism in rats. Water and ethanol extracts were prepared. Three groups of rats received the water extract, ethanol extract, or water by oral gavage for seven days. Pathological section staining of heart tissue. Colorimetric analysis was used to determine serum lactate dehydrogenase. The metabolic expression of small molecules in rats was measured by a metabolomics method. Western blotting was used to detect the expression of phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR), transforming growth factor-β1 (TGF-β1), and caspase-3. Immunohistochemistry was used to detect the expression of CTnI, mTOR, and TGF-β1. The water and ethanol FZ extracts exert cardiotoxic effects via activating the PI3K/Akt/mTOR signaling pathway to induce cardiomyocyte apoptosis. View Full-Text
Keywords: Fuzi; cardiotoxicity; metabolomics; PI3K/Akt/mTOR; caspase-3 Fuzi; cardiotoxicity; metabolomics; PI3K/Akt/mTOR; caspase-3
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Huang, G.; Yang, L.; Zhou, W.; Tang, X.; Wang, Y.; Ma, Z.; Gao, S.; Gao, Y. Study on Cardiotoxicity and Mechanism of “Fuzi” Extracts Based on Metabonomics. Int. J. Mol. Sci. 2018, 19, 3506.

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