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Volume 16
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10.3390/ijms161023143
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Review

Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research

by
Toshitsugu Fujita
and
Hodaka Fujii
*
Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2015, 16(10), 23143-23164; https://doi.org/10.3390/ijms161023143
Submission received: 10 August 2015 / Revised: 31 August 2015 / Accepted: 14 September 2015 / Published: 24 September 2015
(This article belongs to the Special Issue Protein Engineering)
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Abstract

Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species. The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner. In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
Keywords: TAL; TALE; CRISPR/Cas; Cas9; dCas9; sgRNA; transcription; live imaging; locus-specific ChIP; enChIP TAL; TALE; CRISPR/Cas; Cas9; dCas9; sgRNA; transcription; live imaging; locus-specific ChIP; enChIP
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MDPI and ACS Style

Fujita, T.; Fujii, H. Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research. Int. J. Mol. Sci. 2015, 16, 23143-23164. https://doi.org/10.3390/ijms161023143

AMA Style

Fujita T, Fujii H. Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research. International Journal of Molecular Sciences. 2015; 16(10):23143-23164. https://doi.org/10.3390/ijms161023143

Chicago/Turabian Style

Fujita, Toshitsugu, and Hodaka Fujii. 2015. "Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research" International Journal of Molecular Sciences 16, no. 10: 23143-23164. https://doi.org/10.3390/ijms161023143

APA Style

Fujita, T., & Fujii, H. (2015). Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research. International Journal of Molecular Sciences, 16(10), 23143-23164. https://doi.org/10.3390/ijms161023143

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