Constituents from Vigna vexillata and Their Anti-Inflammatory Activity
Abstract
:1. Introduction
2. Results and Discussion
2.1. Purification and Characterization
2.2. Structural Elucidation of New Compounds 1–3
2.3. Anti-Inflammatory Activity
3. Experimental Section
3.1. General
3.2. Plant Materials
3.3. Extraction and Isolation
3.3.1. Vignasterol A (1)
3.3.2. Vigvexin A (2)
3.3.3. Vigvexin B (3)
3.4. Anti-Inflammatory Activity
3.4.1. Preparation of Human Neutrophils
3.4.2. Measurement of Superoxide Anion Generation
3.4.3. Measurement of Elastase Release
3.5. Statistical Analysis
4. Conclusions
Acknowledgments
- Conflict of InterestThe authors have no conflict of interest to report.
References
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Samples | Inhibition Percentage (%) a | |
---|---|---|
Superoxide Anion Generation | Elastase Release | |
methanol extract | 22.06 ± 5.66 * | 19.35 ± 1.52 *** |
chloroform fraction | 57.65 ± 3.69 *** | 67.27 ± 3.53 *** |
water fraction | 11.08 ± 5.19 | 11.00 ± 3.37 * |
Compounds | IC50 (μM) a or (Inh %) b | |
---|---|---|
Superoxide Anion Generation | Elastase Release | |
1 | (12.58 ± 0.82) *** | 8.93 ± 1.64 |
2 | (40.57 ± 4.06) *** | (17.27 ± 4.19) * |
3 | 4.05 ± 0.66 | (12.62 ± 7.17) |
9 | (6.13 ± 3.26) | (−21.93 ± 1.80) *** |
10 | (2.27 ± 2.70) | (−10.96 ± 5.47) |
13 | (15.45 ± 1.17) *** | (11.59 ± 4.53) |
19 | 1.30 ± 0.27 | (42.15 ± 2.88) *** |
20 | (19.26 ± 5.37) * | (11.39 ± 4.98) |
22 | 5.87 ± 0.50 | (19.37 ± 4.16) ** |
23 | 3.13 ± 0.27 | 4.29 ± 0.49 |
25 | 2.66 ± 0.85 | 2.71 ± 0.25 |
27 | (−1.35 ± 3.50) | (16.39 ± 2.85) ** |
30 | (37.34 ± 3.26) *** | (7.68 ± 5.60) |
31 | (20.28 ± 4.96) * | (20.11 ± 2.84) ** |
34 | (26.38 ± 6.94) * | (31.49 ± 5.00) ** |
35 | (26.81 ± 6.19) * | (24.43 ± 4.42) ** |
36 | (17.15 ± 3.77) * | (17.88 ± 1.56) *** |
37 | (8.97 ± 2.64) * | (4.34 ± 0.58) ** |
38 | (−0.88 ± 2.98) | (−1.04 ± 7.31) |
39 | (12.45 ± 5.61) | (−1.30 ± 3.61) |
40 | 4.47 ± 0.76 | 5.51 ± 1.07 |
LY294002 c | 1.38 ± 0.22 | 1.95 ± 0.35 |
DPI d | 0.93 ± 0.52 | – |
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Leu, Y.-L.; Hwang, T.-L.; Kuo, P.-C.; Liou, K.-P.; Huang, B.-S.; Chen, G.-F. Constituents from Vigna vexillata and Their Anti-Inflammatory Activity. Int. J. Mol. Sci. 2012, 13, 9754-9768. https://doi.org/10.3390/ijms13089754
Leu Y-L, Hwang T-L, Kuo P-C, Liou K-P, Huang B-S, Chen G-F. Constituents from Vigna vexillata and Their Anti-Inflammatory Activity. International Journal of Molecular Sciences. 2012; 13(8):9754-9768. https://doi.org/10.3390/ijms13089754
Chicago/Turabian StyleLeu, Yann-Lii, Tsong-Long Hwang, Ping-Chung Kuo, Kun-Pei Liou, Bow-Shin Huang, and Guo-Feng Chen. 2012. "Constituents from Vigna vexillata and Their Anti-Inflammatory Activity" International Journal of Molecular Sciences 13, no. 8: 9754-9768. https://doi.org/10.3390/ijms13089754